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A 3 month old infant has got a white deposition on the mucous membrane of his mouth, tongue and lips. The doctor suspected candidosis. What nutrient medium should be used for inoculation of the material under examination in order to confirm this diagnosis?
- A. Sabouraud
- B. Endo
- C. Loewenstein-Jensen
- D. Roux
Correct Answer: A
Rationale: The correct answer is A: Sabouraud. Sabouraud agar is specifically designed for the cultivation of fungi and yeasts, making it ideal for identifying Candida species causing candidosis. It contains antibiotics to inhibit bacterial growth, promoting the growth of fungi. Endo, Loewenstein-Jensen, and Roux are not suitable for fungal cultures and are used for different purposes like bacterial isolation or mycobacterial cultures. Sabouraud agar is the best choice for confirming candidosis due to its fungal-selective properties.
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The time response measures all of the following except:
- A. ED50
- B. Peak effect time
- C. Onset of response
- D. Threshold level
Correct Answer: A
Rationale: The correct answer is A: ED50. The time response does not measure the ED50 because ED50 refers to the effective dose needed to produce a response in 50% of the population, which is a dose-related concept, not a time-related one. Peak effect time, onset of response, and threshold level are all time-related measures in pharmacology. Peak effect time is the time taken to reach the maximum response, onset of response is the time taken for the response to start, and threshold level is the minimum dose or concentration required to produce a response. Therefore, A is the correct answer as it is the only choice that is not a time-related measure in this context.
A patient with fever and sore throat had a throat culture revealing Gram-positive cocci in chains. The bacteria were catalase-negative and beta-hemolytic. What is the most likely causative agent?
- A. Streptococcus pyogenes
- B. Streptococcus pneumoniae
- C. Enterococcus faecalis
- D. Staphylococcus aureus
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pyogenes.
1. Gram-positive cocci in chains: characteristic of Streptococcus species.
2. Catalase-negative: Streptococcus species are catalase-negative.
3. Beta-hemolytic: S. pyogenes is beta-hemolytic on blood agar.
Therefore, S. pyogenes fits all these criteria.
B: Streptococcus pneumoniae is catalase-negative but alpha-hemolytic, not beta-hemolytic.
C: Enterococcus faecalis is catalase-negative, but not typically beta-hemolytic or in chains.
D: Staphylococcus aureus is catalase-positive and not in chains.
The capsule of Bacillus anthracis is made of
- A. polysaccharide
- B. polypeptide
- C. lipids
- D. they do not form capsules
Correct Answer: B
Rationale: The capsule of Bacillus anthracis is made of polypeptide. This is because the capsule is composed of a protein-based material that surrounds the bacterium, providing protection and aiding in its virulence. Polysaccharides, lipids, and the absence of capsules are not characteristic of Bacillus anthracis' capsule structure, making choices A, C, and D incorrect, respectively.
The cell envelope of Gram-negative bacteria is composed of:
- A. Cytoplasmic membrane, a peptidoglycan layer and an outer membrane
- B. Cytoplasmic membrane and peptidoglycan layer
- C. Only peptidoglycan layer
- D. None of the above
Correct Answer: A
Rationale: The correct answer is A because Gram-negative bacteria have a unique cell envelope structure consisting of a cytoplasmic membrane, a peptidoglycan layer, and an outer membrane. The cytoplasmic membrane is the innermost layer that separates the cytoplasm from the external environment. The peptidoglycan layer provides structural support to the cell. The outer membrane acts as an additional protective barrier and contains lipopolysaccharides. Choice B is incorrect because Gram-negative bacteria have an outer membrane in addition to the cytoplasmic membrane and peptidoglycan layer. Choice C is incorrect because Gram-negative bacteria have more than just a peptidoglycan layer in their cell envelope. Choice D is incorrect as the cell envelope of Gram-negative bacteria does have specific components.
What is the structure of bacterial flagella?
- A. 9+2 microtubule arrangement
- B. protein filaments made of flagellin
- C. lipid bilayer
- D. peptidoglycan layer
Correct Answer: B
Rationale: The correct answer is B: protein filaments made of flagellin. Bacterial flagella are composed of flagellin proteins arranged in helical filaments. This structure allows for movement and propulsion of bacteria.
A: 9+2 microtubule arrangement is incorrect because this structure is typically found in eukaryotic flagella, not bacterial flagella.
C: Lipid bilayer is incorrect as it is a structural component of cell membranes, not bacterial flagella.
D: Peptidoglycan layer is incorrect as it is a component of bacterial cell walls, not flagella.