Nokea
A 6-year-old child with suspected active tuberculosis process has undergone diagnostic Mantoux test. What immunobiological preparation was injected?
- A. Tuberculin
- B. BCG vaccine
- C. DTP vaccine
- D. Tularinum
Correct Answer: A
Rationale: The correct answer is A: Tuberculin. The Mantoux test involves injecting tuberculin, a protein derivative of Mycobacterium tuberculosis, under the skin to check for a delayed hypersensitivity reaction in individuals exposed to TB. B: BCG vaccine is a live attenuated vaccine used for TB prevention, not for diagnostic testing. C: DTP vaccine is for diphtheria, tetanus, and pertussis, not TB. D: Tularinum is not a standard immunobiological preparation used for TB testing. Therefore, A is the correct choice for the Mantoux test.
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During bacteriological examination of sputum of a child with choking cough and fever there were revealed glossy smooth colonies growing on casein-charcoal agar and reminding of mercury drops. Microscopic examination revealed short Gram-negative bacteria. What microorganism was secured from the sputum?
- A. Bordetella pertussis
- B. Haemophylus influenzae
- C. Corynebacterium dyphtheriae
- D. Klebsiella pneumoniae
Correct Answer: A
Rationale: The correct answer is A: Bordetella pertussis. Glossy smooth colonies growing on casein-charcoal agar resembling mercury drops indicate Bordetella pertussis, the causative agent of whooping cough. The presence of short Gram-negative bacteria further supports this identification. Option B, Haemophilus influenzae, typically grows as small, round, translucent colonies on chocolate agar. Option C, Corynebacterium diphtheriae, forms gray colonies on tellurite agar. Option D, Klebsiella pneumoniae, appears as mucoid colonies on MacConkey agar.
The cytoplasmic membrane of the eukaryotes, in contrast to the one of prokaryotes, has:
- A. cellulose
- B. starch
- C. chitin
- D. sterols
Correct Answer: D
Rationale: The correct answer is D: sterols. Eukaryotic cytoplasmic membranes contain sterols, such as cholesterol, which provide stability and regulate fluidity. This is a key characteristic that distinguishes them from prokaryotic membranes. Cellulose (A) is a component of plant cell walls, not the cytoplasmic membrane. Starch (B) is a carbohydrate used for energy storage in plants. Chitin (C) is a component of fungal cell walls and arthropod exoskeletons, not found in eukaryotic cytoplasmic membranes.
A bacteriological analysis revealed spore-forming, Gram-positive rods in a wound smear. The bacteria were motile and produced gas in nutrient broth. What is the causative agent?
- A. Clostridium perfringens
- B. Bacillus anthracis
- C. Clostridium tetani
- D. Bacillus cereus
Correct Answer: A
Rationale: Rationale:
1. Spore-forming, Gram-positive rods: Characteristics shared by Clostridium and Bacillus.
2. Motile and gas production in nutrient broth: Clostridium perfringens is motile and produces gas, distinguishing it from Bacillus species.
3. Clostridium perfringens causes gas gangrene, correlating with gas production.
4. Bacillus anthracis causes anthrax, not related to wound infections.
5. Clostridium tetani causes tetanus, not associated with gas production.
6. Bacillus cereus causes food poisoning, not commonly associated with wound infections.
Summary:
- A is correct (Clostridium perfringens) due to motility, gas production, and wound infection correlation.
- B (Bacillus anthracis) causes anthrax, not wound infections.
- C (Clostridium tetani) causes tetanus, not gas production.
- D (B
A man is ill with a protozoan disease characterized by cerebral affection and loss of sight. Blood analysis revealed halfmoon-shaped unicellular organisms with pointed ends. This disease is caused by:
- A. Toxoplasma B.Leishmania C.Lamblia D.Amoeba E.Trichomonad
Correct Answer: A
Rationale: The correct answer is A: Toxoplasma. Toxoplasma gondii causes Toxoplasmosis, a protozoan disease that can affect the brain and lead to vision loss. The halfmoon-shaped unicellular organisms with pointed ends described in the blood analysis are characteristic of Toxoplasma parasites. Toxoplasma infection commonly occurs through ingestion of contaminated food or water, or contact with infected cat feces. Leishmania causes Leishmaniasis, Lamblia causes Giardiasis, Amoeba causes Amebiasis, and Trichomonad causes Trichomoniasis, none of which match the symptoms and blood analysis findings described in the question.
Laboratory diagnosis of Rotavirus infection includes
- A. Egg inoculation
- B. Sensitive laboratory animals
- C. Ascoli test
- D. Cell cultures
Correct Answer: D
Rationale: The correct answer is D: Cell cultures. Rotavirus diagnosis involves detecting viral antigens in stool samples using cell cultures. Cell cultures allow for the replication of the virus, facilitating its identification. A: Egg inoculation and B: Sensitive laboratory animals are not commonly used for Rotavirus diagnosis due to ethical concerns and practical limitations. C: Ascoli test is used for detecting specific proteins in serum, not for Rotavirus detection in stool samples. In summary, cell cultures are the most appropriate method for laboratory diagnosis of Rotavirus infection due to their ability to support viral replication and antigen detection.