Nokea
How is viral multiplication detected in cell culture?
- A. by the presence of a specific cytopathic effect
- B. by using an immunofluorescence detection method
- C. the characteristic type of viral colonies
- D. A and B both
Correct Answer: D
Rationale: The correct answer is D because viral multiplication in cell culture can be detected by observing a specific cytopathic effect (A) caused by viral infection in cells and by using an immunofluorescence detection method (B) to visualize viral antigens within the cells. Choice C, the characteristic type of viral colonies, is incorrect as viral multiplication is not typically detected by observing viral colonies in cell culture. Option D is the correct choice as both A and B methods are commonly used to detect viral multiplication in cell culture.
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A patient has food poisoning. Laboratory analysis revealed a culture of anaerobic gram-positive spore-forming bacteria. What is the most likely kind of the isolated causative agent?
- A. C. perfringens
- B. Proteus vulgaris
- C. P. mirabilis
- D. Vibrio parahemolyticus
Correct Answer: A
Rationale: The correct answer is A: C. perfringens. This is because Clostridium perfringens is a gram-positive, anaerobic, spore-forming bacterium commonly associated with food poisoning. It produces toxins that cause gastrointestinal symptoms. Proteus vulgaris (B) and P. mirabilis (C) are gram-negative bacteria not typically associated with food poisoning. Vibrio parahemolyticus (D) is a gram-negative bacterium causing seafood-related gastroenteritis, not spore-forming or anaerobic.
Patients with similar complaints applied to the doctor: weakness, pain in the intestines, disorder of GIT. Examination of the feces revealed that one patient with four nucleus cysts should be hospitalized immediately. For what protozoa are such cysts typical?
- A. Lamblia
- B. Dysenteric amoeba
- C. Balantidium
- D. Trichomonas
Correct Answer: B
Rationale: The correct answer is B: Dysenteric amoeba.
1. Dysenteric amoeba causes amoebiasis, which presents with symptoms like weakness, intestinal pain, and gastrointestinal disorders.
2. The presence of four-nucleus cysts in the feces indicates an infection with Entamoeba histolytica, the causative agent of amoebiasis.
3. Multiple-nucleus cysts are characteristic of Entamoeba histolytica, distinguishing it from other protozoa.
4. Lamblia (choice A), Balantidium (choice C), and Trichomonas (choice D) are not associated with four-nucleus cysts or the mentioned symptoms, making them incorrect choices.
In which of the following GIT infections, the bacteria adhere to the intestine wall and produce toxins
- A. Shigella spp.
- B. Salmonella spp.
- C. enterotoxigenic E. Coli (ETEC)
- D. Helicobacter pylori
Correct Answer: C
Rationale: The correct answer is C: enterotoxigenic E. Coli (ETEC). ETEC adheres to the intestine wall and produces toxins, leading to symptoms like diarrhea. Shigella spp. invade intestinal cells, Salmonella spp. cause inflammation, and Helicobacter pylori infects the stomach lining. This makes them incorrect choices for bacteria adhering to the intestine wall and producing toxins.
A patient with a productive cough and fever had a sputum smear revealing Gram-positive cocci in clusters. What is the most likely causative agent?
- A. Staphylococcus aureus
- B. Streptococcus pneumoniae
- C. Neisseria meningitidis
- D. Klebsiella pneumoniae
Correct Answer: A
Rationale: The correct answer is A: Staphylococcus aureus. Staphylococcus aureus is a Gram-positive cocci bacterium that forms clusters and commonly causes pneumonia with a productive cough and fever. It is frequently found in sputum smears in such cases. Streptococcus pneumoniae (B) is another common cause of pneumonia but typically appears in pairs or chains, not clusters. Neisseria meningitidis (C) is a Gram-negative diplococcus that causes meningitis, not pneumonia. Klebsiella pneumoniae (D) is a Gram-negative bacterium that can cause pneumonia but appears as Gram-negative rods, not Gram-positive cocci in clusters.
Which scientist performed the first successful vaccination against smallpox?
- A. Pasteur
- B. Jenner
- C. Semmelweis
- D. Koch
Correct Answer: B
Rationale: The correct answer is B: Jenner. Edward Jenner is credited with performing the first successful vaccination against smallpox in 1796. He used cowpox virus to inoculate individuals, leading to immunity against smallpox. Jenner's experiment laid the foundation for modern vaccination. Pasteur (A) is known for developing the germ theory of disease. Semmelweis (C) advocated for handwashing to prevent infections. Koch (D) identified the causative agents of several diseases but did not develop the smallpox vaccine.