Nokea
How the prepared immunofluorescence slide should be examined?
- A. Under the fluorescence microscope using x100 oil immersion objective
- B. Under light microscope using x100 oil immersion objective
- C. Using electron microscopy
- D. None of the above
Correct Answer: A
Rationale: The correct answer is A because immunofluorescence slides are examined under a fluorescence microscope with a x100 oil immersion objective. This is because immunofluorescence relies on the detection of fluorescently labeled antibodies, which emit light when excited by a specific wavelength. Using a fluorescence microscope allows for visualization of these labeled antibodies at high magnification and resolution, enabling precise examination of cellular structures and protein localization. Choice B is incorrect as light microscopes are not suitable for visualizing fluorescence. Choice C is incorrect as electron microscopes use electrons, not light, for imaging, which is not compatible with immunofluorescence. Choice D is incorrect as there is a specific method for examining immunofluorescence slides.
You may also like to solve these questions
A patient with a deep tissue infection had Gram-negative rods isolated from the wound culture. The bacteria were oxidase-positive and non-lactose fermenters. What is the most likely causative agent?
- A. Pseudomonas aeruginosa
- B. Escherichia coli
- C. Klebsiella pneumoniae
- D. Shigella dysenteriae
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. This is because Pseudomonas is a Gram-negative rod, oxidase-positive, and non-lactose fermenter. These characteristics are typical of Pseudomonas species.
B: Escherichia coli is a Gram-negative rod but is lactose fermenter and usually oxidase-negative.
C: Klebsiella pneumoniae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative.
D: Shigella dysenteriae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative and is more associated with causing shigellosis rather than deep tissue infections.
From a medicinal herb a certain phytopathogenic microorganism was secured. In the nutrient medium it forms "fried egg"colonies. What is the most likely agent?
- A. Mycoplasma B.Yeasts C.Actinomycetes D.Nocardia E.Pseudomonas Legionella
Correct Answer: C
Rationale: Step 1: "Fried egg" colonies typically refer to colonies with a raised center and flattened periphery, resembling the appearance of a fried egg.
Step 2: Actinomycetes are filamentous bacteria known to form "fried egg" colonies in nutrient medium.
Step 3: Actinomycetes are commonly found in soil and are known to be phytopathogenic microorganisms.
Step 4: Actinomycetes produce secondary metabolites with medicinal properties, making them a likely agent from a medicinal herb.
Overall, the correct answer is C (Actinomycetes) as they fit the description provided in the question and are commonly associated with the given scenario. The other choices are incorrect as they do not exhibit the specific characteristics mentioned in the question or are not typically associated with forming "fried egg" colonies in nutrient medium.
A patient with a sore throat and fever had a throat smear stained with Gram's method, revealing Gram-positive cocci in chains. What is the most likely causative agent?
- A. Streptococcus pyogenes
- B. Staphylococcus aureus
- C. Neisseria gonorrhoeae
- D. Enterococcus faecalis
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pyogenes. This bacterium is known to cause sore throat and fever, commonly referred to as strep throat. Gram's stain showing Gram-positive cocci in chains is characteristic of Streptococcus species. Streptococcus pyogenes is a beta-hemolytic bacterium that forms chains and is a common cause of bacterial pharyngitis. Staphylococcus aureus (B) is a Gram-positive cocci that forms clusters, not chains. Neisseria gonorrhoeae (C) is a Gram-negative diplococcus and is not associated with sore throat. Enterococcus faecalis (D) is a Gram-positive cocci that can cause infections but is not typically associated with sore throat.
Which of the following is responsible for causing gonorrhea?
- A. Neisseria gonorrhoeae
- B. Chlamydia trachomatis
- C. Mycobacterium tuberculosis
- D. Treponema pallidum
Correct Answer: A
Rationale: Neisseria gonorrhoeae is the correct answer because it is the specific bacterium responsible for causing gonorrhea. It is a sexually transmitted infection that primarily affects the genital tract. Neisseria gonorrhoeae is transmitted through sexual contact and can lead to symptoms such as painful urination, discharge, and pelvic pain.
Chlamydia trachomatis (Choice B) is another sexually transmitted bacterium, but it causes chlamydia, not gonorrhea.
Mycobacterium tuberculosis (Choice C) is responsible for tuberculosis, a different infectious disease that primarily affects the lungs.
Treponema pallidum (Choice D) causes syphilis, another sexually transmitted infection that presents with distinct symptoms from gonorrhea.
Therefore, Neisseria gonorrhoeae is the correct choice for causing gonorrhea due to its specific association with this particular infection.
The flagellar antigen of the bacteria is known as:
- A. D antigen
- B. H antigen
- C. K antigen
- D. F antigen
Correct Answer: B
Rationale: The correct answer is B: H antigen. Flagellar antigen is typically referred to as the H antigen in bacteria. Flagella are the whip-like structures used for movement in bacteria, and the H antigen is specifically associated with the flagella. The other choices (A: D antigen, C: K antigen, D: F antigen) are not related to flagella in bacteria and are commonly associated with other types of antigens in bacterial classification.