microbiology an introduction 12th edition test bank Related

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Microbiological analysis of medicinal raw materials revealed capsular bacteria. What stain method was used to detect the capsules?

  • A. Gin's
  • B. Ziehl-Neelsen's
  • C. Neisser's
  • D. Gram's
Correct Answer: A

Rationale: The correct answer is A: Gin's stain method. Gin's stain method is specifically used to detect capsules of bacteria. This method involves staining the bacteria with crystal violet and copper sulfate, which highlights the capsules as a clear halo around the stained bacterial cells. Ziehl-Neelsen's stain is used for acid-fast bacteria like Mycobacterium tuberculosis, Neisser's stain is for detecting Neisseria species, and Gram's stain is for differentiating bacteria based on their cell wall composition (Gram-positive vs. Gram-negative). Therefore, choices B, C, and D are incorrect for this scenario.