Nokea
When using gram staining method fungi stain:
- A. Blue-purple
- B. Red
- C. Silver
- D. Do not stain
Correct Answer: D
Rationale: The correct answer is D: Fungi do not stain in the gram staining method because they have a unique cell wall composition different from bacteria. Fungi have chitin in their cell walls, which is not easily stained by the gram staining process. This method primarily targets the peptidoglycan layer present in bacterial cell walls. Therefore, fungi will not take up the stain and will remain colorless. The other choices (A: Blue-purple, B: Red, C: Silver) are incorrect because they describe colors that are associated with staining results for bacteria, not fungi.
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When a smear is stained by Burri-Gins method a mucous structure that is tightly bound with the cellular wall of bacteria and has well-defined outer boundaries can be detected. This element of a bacteria cell is called:
- A. Capsule
- B. Spore
- C. Filaments
- D. Ribosomes
Correct Answer: A
Rationale: The correct answer is A: Capsule. A capsule is a mucous structure tightly bound to the bacterial cell wall with distinct outer boundaries. This structure helps bacteria evade the host immune system, resist desiccation, and adhere to surfaces. In contrast, spores are resistant structures formed by certain bacteria for survival, filaments are long chains of bacterial cells, and ribosomes are cellular organelles responsible for protein synthesis. Therefore, based on the characteristics described in the question, the most appropriate choice is A: Capsule.
Which of the following transport mechanisms transports water across the plasma membrane?
- A. osmosis
- B. facilitated diffusion
- C. phagocytosis
- D. exocytosis
Correct Answer: A
Rationale: Osmosis is the correct answer because it is the process by which water molecules move across a semi-permeable membrane from an area of low solute concentration to an area of high solute concentration. This movement occurs passively, driven by the concentration gradient. Facilitated diffusion (B) involves the transport of specific molecules with the help of carrier proteins, not water. Phagocytosis (C) is a process where cells engulf solid particles, not water molecules. Exocytosis (D) is the process of releasing substances from a cell by fusion of vesicles with the cell membrane, not the transport of water across the membrane.
Prophylaxis of smallpox and revaccination is performed:
- A. Every year after the first vaccine
- B. Every second year
- C. Every three years
- D. No revaccination is needed, just the initial vaccine is enough
Correct Answer: D
Rationale: The correct answer is D because smallpox vaccination provides long-lasting immunity, often for a lifetime. Revaccination is not necessary as the initial vaccine confers sufficient protection. Choice A is incorrect because yearly revaccination is unnecessary and excessive. Choice B and C are also incorrect as there is no need for revaccination every second or third year. The key is to understand that smallpox vaccination typically provides lifelong immunity, making ongoing revaccination unnecessary.
Microscopic examination of a smear from a chancre revealed spiral microorganisms. The bacteria were thin and mobile with 8-12 regular coils. What is the likely diagnosis?
- A. Syphilis
- B. Leptospirosis
- C. Lyme disease
- D. Relapsing fever
Correct Answer: A
Rationale: The correct diagnosis is A: Syphilis. The spiral microorganisms described are characteristic of Treponema pallidum, the causative agent of syphilis. The thin and mobile bacteria with 8-12 regular coils match the morphology of Treponema pallidum. Leptospirosis is caused by Leptospira interrogans, which are thicker and have hook-like ends, different from the described bacteria. Lyme disease is caused by Borrelia burgdorferi, which has a different appearance than the thin, coiled bacteria in the smear. Relapsing fever is caused by Borrelia recurrentis, which typically have irregular coils, unlike the regular coils seen in the smear.
During sanitary and bacteriological testing of water with the membrane filter technique there were revealed two red colonies on a membrane filter (Endo agar) through which 500 ml of water was filtred. Calculate the coli index and coli titer of the analyzed water:
- A. 4 and 250
- B. 2 and 500
- C. 250 and 4
- D. 500 and 2
Correct Answer: C
Rationale: The correct answer is C (coli index of 250 and coli titer of 4). The coli index is calculated by multiplying the number of colonies by the reciprocal of the volume filtered, which gives 2 colonies x (1/0.5 L) = 4. The coli titer is obtained by dividing the volume filtered by the number of colonies, which gives 0.5 L / 2 colonies = 0.25 L or 250 ml. This indicates the concentration of coliform bacteria in the water sample.
Choice A (4 and 250) is incorrect because it has the correct coli index but the values are reversed for coli index and coli titer. Choice B (2 and 500) is incorrect as it provides the correct coli titer value but the values are reversed for coli index and coli titer. Choice D (500 and 2) is incorrect because it reverses the values for both coli index and coli titer.