What causes the changes in appearance seen in boys at the time of puberty?
Correct Answer: D
Rationale: During puberty, boys experience physical changes such as growth of facial and body hair, deepening of the voice, and muscle development. These changes are primarily driven by the hormone testosterone. Testosterone is responsible for the development of male secondary sexual characteristics. Therefore, the correct answer is testosterone. Choices A, B, and C are incorrect because prolactin, adrenaline, and estrogen do not play a significant role in the development of male secondary sexual characteristics during puberty.