Most reliable test for detection of acute hepatitis A infection is
- A. Western blot assay for IgG anti-HAV in serum
- B. ELISA test for IgM anti-HAV in serum and HAV-Ag in stools
- C. ELISA test for IgG anti-HAV in serum
- D. None of the above
Correct Answer: B
Rationale: The correct answer is B: ELISA test for IgM anti-HAV in serum and HAV-Ag in stools. This test is the most reliable for detecting acute hepatitis A infection because IgM antibodies are produced early in the infection, indicating an active infection. Detection of HAV antigen in stools also confirms active viral shedding.
A: Western blot assay for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
C: ELISA test for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
D: None of the above is incorrect as option B is the most appropriate choice for detecting acute hepatitis A infection.
You may also like to solve these questions
In an agar slant, bacterial colonies that appear separate or semiconfluent are referred to as:
- A. filiform
- B. echinulate
- C. beaded
- D. effuse
Correct Answer: C
Rationale: The correct answer is C: beaded. In an agar slant, beaded colonies appear as separate or semiconfluent, forming small, round colonies with gaps between them. This term accurately describes the appearance of the colonies in this context.
A: Filiform colonies are dense and opaque with smooth edges, not separate or semiconfluent.
B: Echinulate colonies have spiny projections, not the characteristic of separate or semiconfluent growth.
D: Effuse colonies spread out extensively and lack defined borders, unlike the distinct appearance of separate or semiconfluent colonies.
Which bacteria is known for causing the disease cholera?
- A. Shigella dysenteriae
- B. Vibrio cholerae
- C. Salmonella enterica
- D. Clostridium perfringens
Correct Answer: B
Rationale: The correct answer is B: Vibrio cholerae. Cholera is caused by Vibrio cholerae, a gram-negative bacterium that produces a toxin leading to severe watery diarrhea. Shigella dysenteriae causes dysentery, not cholera. Salmonella enterica causes salmonellosis, not cholera. Clostridium perfringens is associated with food poisoning, not cholera. In summary, Vibrio cholerae is the correct answer because it is the specific bacterium known to cause cholera, while the other choices are associated with different diseases.
A child is presumably ill with diphtheria. A specimen of affected mucous membrane of his pharynx was taken for analysis. The smear was stained and microscopic examination revealed yellow rods with dark blue thickenings on their ends. What structural element of a germ cell was revealed in the detected microorganisms?
- A. Volutin granules
- B. Plasmids
- C. Capsule
- D. Spores
Correct Answer: A
Rationale: The correct answer is A: Volutin granules. Volutin granules are metachromatic granules that stain dark blue with methylene blue or Leishman stain. In diphtheria-causing bacteria, Corynebacterium diphtheriae, these granules are typically seen as yellow rods with dark blue thickenings on their ends when stained. Volutin granules are storage inclusions composed of polymetaphosphate that serve as an energy reserve for the bacteria. The presence of these granules in the detected microorganisms indicates the characteristic feature of Corynebacterium diphtheriae.
Summary of other choices:
B: Plasmids are extrachromosomal DNA molecules that are not typically visible on stained smears.
C: Capsules are outer protective layers that are not visible on stained smears.
D: Spores are reproductive structures formed by certain bacteria, not typically seen in Corynebacterium diphtheriae.
Sanitary assessment of a pond, where the children from a recreation summer camp take their swims, detected there oval cysts 50-60 micron in diameter, with 2 nuclei visible in their cytoplasm (macronucleus and micronucleus). What protozoa do these cysts belong to?
- A. Toxoplasma
- B. Amoeba
- C. Balantidium
- D. Lamblia
Correct Answer: D
Rationale: The correct answer is D: Lamblia. The cysts described match the characteristics of Giardia lamblia, a protozoan parasite. Giardia cysts are oval-shaped, 50-60 microns in diameter, and contain 2 nuclei (macronucleus and micronucleus). Giardia is commonly found in contaminated water sources and can cause gastrointestinal illness in humans.
Incorrect choices:
A: Toxoplasma - Toxoplasma gondii is a parasite that causes toxoplasmosis, transmitted through ingestion of contaminated food or water, but its cysts do not match the description given in the question.
B: Amoeba - Amoebas are single-celled organisms, but they do not typically form cysts with visible nuclei as described.
C: Balantidium - Balantidium coli is a ciliated protozoan that can cause dysentery in humans, but its cysts do not
A patient with pulmonary tuberculosis is prescribed the most effective antituberculosis antibiotic. Name this drug:
- A. Tetracycline
- B. Furasolidone
- C. Rifampicin
- D. Bactrim (Co-trimoxazole)
Correct Answer: C
Rationale: Rifampicin is the correct answer because it is a first-line antibiotic for treating pulmonary tuberculosis. It has excellent bactericidal activity and penetrates well into lung tissues where the infection is located. Rifampicin is also known for preventing the development of resistance. Tetracycline (A) and Furasolidone (B) are not commonly used for tuberculosis treatment. Bactrim (D) is a combination of sulfamethoxazole and trimethoprim, not effective for tuberculosis treatment.