Mastering Microbiology Test Bank

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A bacteriological laboratory received sputum sample of a patient suffering from tuberculosis. Bacterioscopic examination of smears and detection of tuberculosis bacillus can be realized by one of enrichment methods that involves processing of sputum only with solution of caustic soda. What is this method called?

  • A. Homogenization
  • B. Inactivation
  • C. Flotation
  • D. Filtration
Correct Answer: B

Rationale: The correct answer is B: Inactivation. In this method, the sputum sample is processed with a solution of caustic soda, which helps to kill any contaminants present and preserve the tuberculosis bacillus for microscopic examination. This step is crucial in ensuring accurate detection of the bacillus.

A: Homogenization involves breaking down the sample to make it uniform, but it does not necessarily involve using caustic soda for inactivation.
C: Flotation is a method used to separate different components based on their density and is not specific to inactivating tuberculosis bacillus.
D: Filtration involves passing a sample through a filter to separate particles based on size and is not related to inactivating the tuberculosis bacillus.

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Which of the following pathogens has the widest infectious spectrum

  • A. Anthrax bacillus
  • B. mumps virus
  • C. the causative agent of gonorrhea
  • D. the rabies virus
Correct Answer: A

Rationale: The correct answer is A, Anthrax bacillus, because it has the widest infectious spectrum among the options provided. Anthrax bacillus can infect a wide range of hosts, including humans and various animals. It is known for its ability to survive in different environments and cause disease through various routes of exposure. In contrast, choices B, C, and D have more limited infectious spectra. Mumps virus primarily infects humans, the causative agent of gonorrhea mainly affects humans through sexual contact, and the rabies virus typically infects mammals through bites from infected animals. Therefore, Anthrax bacillus is the correct choice due to its broader range of hosts and modes of transmission compared to the other options.

The main function of bacterial ribosomes is:

  • A. DNA replication
  • B. protein synthesis
  • C. cell wall synthesis
  • D. RNA splicing
Correct Answer: B

Rationale: The correct answer is B: protein synthesis. Bacterial ribosomes are responsible for translating messenger RNA into proteins through a process called translation. Ribosomes are composed of both protein and RNA molecules and facilitate the binding of transfer RNA to the messenger RNA to assemble the amino acids in the correct order to form a protein. DNA replication (A) is carried out by enzymes such as DNA polymerase, not ribosomes. Cell wall synthesis (C) is a process involving enzymes that build the structure of the cell wall in bacteria. RNA splicing (D) is a eukaryotic process that involves removing introns from pre-mRNA molecules, which is not a function of bacterial ribosomes.

An inflammatory disease that can develop as a complication following a group A streptococcal infection is:

  • A. endocarditis
  • B. rheumatic fever
  • C. nephritis
  • D. cytomegalovirus
Correct Answer: B

Rationale: The correct answer is B: rheumatic fever. Rheumatic fever is an inflammatory disease that can occur as a complication following a group A streptococcal infection. This is known as a post-streptococcal complication. It typically affects the heart, joints, skin, and brain. Endocarditis (choice A) is also a complication of streptococcal infections, but it is an infection of the heart valves, not an inflammatory disease like rheumatic fever. Nephritis (choice C) is inflammation of the kidneys and is not directly related to streptococcal infections. Cytomegalovirus (choice D) is a different viral infection and is not associated with streptococcal infections.

Slime, blood and protozoa 30-200 microns of length have been revealed in a man's feces. The body is covered with cilias and has correct oval form with a little bit narrowed forward and wide round shaped back end. On the forward end a mouth is visible. In cytoplasm there are two nucleuses and two short vacuoles. For whom are the described attributes typical?

  • A. Balantidium
  • B. Lamblia
  • C. Dysenteric amoeba
  • D. Trichomonas
Correct Answer: A

Rationale: Rationale: The described attributes match those of Balantidium. The presence of cilia, oval shape with a narrowed front and wide back end, visible mouth, two nucleuses, and short vacuoles are all characteristic features of Balantidium. Balantidium is a ciliated protozoan parasite commonly found in the human intestine. The other choices can be eliminated based on specific characteristics: Lamblia lacks cilia and has a different shape, Dysenteric amoeba typically has a single nucleus, and Trichomonas does not have cilia or multiple nuclei. Therefore, based on the specific attributes described, the correct answer is Balantidium (Choice A).

Which of the following is a folate antagonist?

  • A. Methyl Penicillin
  • B. Trimethoprim
  • C. Vancomycin
  • D. Gentamicin
Correct Answer: B

Rationale: The correct answer is B: Trimethoprim. Trimethoprim is a folate antagonist that inhibits dihydrofolate reductase, an enzyme essential for folate metabolism in bacteria. This disrupts bacterial DNA synthesis, leading to bacteriostatic effects. Methyl Penicillin (A) is a beta-lactam antibiotic that interferes with bacterial cell wall synthesis. Vancomycin (C) inhibits cell wall synthesis by binding to D-Ala-D-Ala terminus of cell wall precursors. Gentamicin (D) inhibits bacterial protein synthesis by binding to the 30S subunit of the bacterial ribosome.

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