Babesiosis is a vector-borne, malaria-like illness caused by a:
- A. bacterium
- B. virus
- C. helminth
- D. protozoan
Correct Answer: D
Rationale: The correct answer is D: protozoan. Babesiosis is caused by a protozoan parasite called Babesia. Protozoans are single-celled organisms that can cause various diseases in humans. Babesiosis is transmitted to humans through the bite of infected ticks. Bacteria (choice A), viruses (choice B), and helminths (choice C) are not responsible for causing babesiosis. Bacteria are typically responsible for diseases like Lyme disease, viruses for illnesses like Zika, and helminths for infections such as parasitic worms. Thus, the correct choice for the causative agent of babesiosis is a protozoan.
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The causative agent of syphilis is:
- A. Borrelia burgdorferi
- B. Treponema pallidum
- C. Leptospira interrogans
- D. Rickettsia rickettsii
Correct Answer: B
Rationale: The correct answer is B: Treponema pallidum.
1. Treponema pallidum is a spirochete bacterium known to cause syphilis.
2. Borrelia burgdorferi causes Lyme disease, not syphilis.
3. Leptospira interrogans causes leptospirosis, not syphilis.
4. Rickettsia rickettsii causes Rocky Mountain spotted fever, not syphilis.
Therefore, Treponema pallidum is the causative agent of syphilis, making it the correct answer.
The most common congenital infection worldwide is due to:
- A. CMV
- B. Listeria monocytogenes
- C. Rubella Virus
- D. Syphilis
Correct Answer: A
Rationale: The correct answer is A: CMV (Cytomegalovirus). CMV is the most common congenital infection worldwide due to its high prevalence and ability to be transmitted from mother to fetus during pregnancy. It can lead to serious complications such as hearing loss, developmental delays, and vision problems in newborns. Listeria monocytogenes (B) is a foodborne pathogen, not typically associated with congenital infections. Rubella Virus (C) can cause congenital rubella syndrome but is less common globally compared to CMV. Syphilis (D) can also be transmitted from mother to fetus, but its prevalence and impact on congenital infections are lower compared to CMV.
Specimen of a patient's sputum was stained with the following dyes and reagents: Ziehl's solution, methylene blue solution, 5% solutoin of sulfuric acid. What staining method was applied?
- A. Ziehl-Neelsen
- B. Burri's
- C. Gram's
- D. Peshkov's
Correct Answer: A
Rationale: The correct answer is A: Ziehl-Neelsen staining method was applied.
1. Ziehl's solution is used as a primary stain in Ziehl-Neelsen staining for acid-fast organisms like Mycobacterium tuberculosis.
2. Sulfuric acid is used as a decolorizer to remove excess stain.
3. Methylene blue is not part of the Ziehl-Neelsen staining method.
4. Burri's, Gram's, and Peshkov's staining methods are not used for acid-fast staining.
Which of the following microorganisms are acid-fast?
- A. Streptococcus pyogenes
- B. Mycobacterium tuberculosis
- C. Clostridium perfringens
- D. Bacillus subtilis
Correct Answer: B
Rationale: The correct answer is B: Mycobacterium tuberculosis. Acid-fast bacteria have a unique cell wall component called mycolic acid, making them resistant to decolorization by acid-alcohol during staining. Mycobacterium tuberculosis is acid-fast, as it retains the stain due to its high lipid content. Streptococcus pyogenes (A), Clostridium perfringens (C), and Bacillus subtilis (D) do not possess mycolic acid in their cell walls, so they are not acid-fast.
A laboratory received a sample of water used in drug production for sanitary and viral analysis. What group of viruses will indicate fecal contamination of water and thus the need for its additional purification?
- A. Picornaviridae
- B. Herpesviridae
- C. Flaviviridae
- D. Retroviridae
Correct Answer: A
Rationale: The correct answer is A: Picornaviridae. Picornaviruses, such as enteroviruses, are commonly found in the feces of infected individuals. Therefore, their presence in water indicates fecal contamination and the need for additional purification.
B: Herpesviridae are not typically associated with fecal contamination.
C: Flaviviridae are mainly transmitted through arthropods like mosquitoes, not fecal contamination.
D: Retroviridae, including HIV, are not typically excreted in high levels in feces and are not indicators of fecal contamination.