Nokea
From a medicinal herb a certain phytopathogenic microorganism was secured. In the nutrient medium it forms "fried egg"colonies. What is the most likely agent?
- A. Mycoplasma B.Yeasts C.Actinomycetes D.Nocardia E.Pseudomonas Legionella
Correct Answer: C
Rationale: Step 1: "Fried egg" colonies typically refer to colonies with a raised center and flattened periphery, resembling the appearance of a fried egg.
Step 2: Actinomycetes are filamentous bacteria known to form "fried egg" colonies in nutrient medium.
Step 3: Actinomycetes are commonly found in soil and are known to be phytopathogenic microorganisms.
Step 4: Actinomycetes produce secondary metabolites with medicinal properties, making them a likely agent from a medicinal herb.
Overall, the correct answer is C (Actinomycetes) as they fit the description provided in the question and are commonly associated with the given scenario. The other choices are incorrect as they do not exhibit the specific characteristics mentioned in the question or are not typically associated with forming "fried egg" colonies in nutrient medium.
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Which of the following microorganisms can be cultured only in the footpads of mice or a species of armadillo?
- A. Mycobacterium tuberculosis
- B. Mycobacterium leprae
- C. Neisseria gonorrhoeae
- D. Mycoplasma
Correct Answer: B
Rationale: The correct answer is B: Mycobacterium leprae. This microorganism can only be cultured in the footpads of mice or a species of armadillo due to its specific growth requirements. Mycobacterium tuberculosis (choice A) can be cultured in various culture media in the laboratory. Neisseria gonorrhoeae (choice C) can be cultured on selective media like Thayer-Martin agar. Mycoplasma (choice D) can be cultured in cell cultures or specialized media. Therefore, the unique growth pattern of Mycobacterium leprae makes it the correct answer.
While studying a microslide obtained from the punctuate of a regional lymph node and stained by Romanovsky-Giemsa method a physician revealed some light pink thin microorganisms with 12-14 regular spiral coils and pointed ends, up to 10-13 micrometer long. This might be the causative agent of the following disease:
- A. Syphilis
- B. Trypanosomiasis
- C. Leptospirosis
- D. Relapsing fever
Correct Answer: D
Rationale: The correct answer is D: Relapsing fever. The description matches Borrelia species, the causative agent of relapsing fever. The thin, spiral-shaped microorganisms with pointed ends and specific length are characteristic of Borrelia. The number of coils and size mentioned also align with Borrelia morphology. Relapsing fever is transmitted by tick bites and manifests as recurring febrile episodes.
Incorrect choices:
A: Syphilis - Syphilis is caused by Treponema pallidum, which is not spiral-shaped.
B: Trypanosomiasis - Trypanosomiasis is caused by Trypanosoma species, which are not spiral-shaped.
C: Leptospirosis - Leptospirosis is caused by Leptospira species, which are not spiral-shaped and do not match the specific description provided.
During the skill-building session in microbiology the students need to stain the prepared and fixed sputum smears obtained from a tuberculosis patient. What staining technique should be used in this case?
- A. Gram
- B. Giemsa
- C. Ziehl-Neelsen
- D. Burry
Correct Answer: C
Rationale: The correct staining technique for staining sputum smears from a tuberculosis patient is Ziehl-Neelsen staining. This technique uses a carbol fuchsin stain that specifically stains acid-fast organisms like Mycobacterium tuberculosis. The steps involved include staining with carbol fuchsin, decolorizing with acid-alcohol, counterstaining with methylene blue, and then examining under a microscope. This staining method is crucial for detecting the acid-fast bacilli characteristic of tuberculosis.
Explanation for incorrect choices:
A: Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative, not suitable for acid-fast organisms like Mycobacterium tuberculosis.
B: Giemsa staining is used for detecting parasites and certain types of white blood cells, not specific for acid-fast bacteria.
D: Burry staining is not a recognized staining technique in microbiology for staining acid-fast organisms.
Gram-positive cocci arranged in chains were found in a wound smear. The bacteria were catalase-negative and beta-hemolytic on blood agar. What is the causative agent?
- A. Streptococcus pyogenes
- B. Staphylococcus aureus
- C. Enterococcus faecalis
- D. Micrococcus luteus
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pyogenes. This bacterium fits all the given characteristics: Gram-positive cocci arranged in chains, catalase-negative, and beta-hemolytic on blood agar. Streptococcus pyogenes is known for causing skin and soft tissue infections. Staphylococcus aureus (B) is catalase-positive and typically forms clusters, not chains. Enterococcus faecalis (C) is catalase-negative but not beta-hemolytic. Micrococcus luteus (D) is catalase-positive and not typically associated with wound infections.
A patient with a sore throat and fever had a throat smear stained with Gram's method, revealing Gram-positive cocci in chains. What is the most likely causative agent?
- A. Streptococcus pyogenes
- B. Staphylococcus aureus
- C. Neisseria gonorrhoeae
- D. Enterococcus faecalis
Correct Answer: A
Rationale: The correct answer is A: Streptococcus pyogenes. This bacterium is known to cause sore throat and fever, commonly referred to as strep throat. Gram's stain showing Gram-positive cocci in chains is characteristic of Streptococcus species. Streptococcus pyogenes is a beta-hemolytic bacterium that forms chains and is a common cause of bacterial pharyngitis. Staphylococcus aureus (B) is a Gram-positive cocci that forms clusters, not chains. Neisseria gonorrhoeae (C) is a Gram-negative diplococcus and is not associated with sore throat. Enterococcus faecalis (D) is a Gram-positive cocci that can cause infections but is not typically associated with sore throat.