Reproductive System Anatomy Quizlet Related

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If a radioimmunoassay is properly conducted and the amount of radioactive hormone bound to antibody is low, this would indicate which of the following?

  • A. Plasma levels of endogenous hormone are high
  • B. Plasma levels of endogenous hormone are low
  • C. More antibody is needed
  • D. Less radioactive hormone is needed
Correct Answer: A

Rationale: The correct answer is A: Plasma levels of endogenous hormone are high. When the amount of radioactive hormone bound to the antibody is low in a radioimmunoassay, it indicates that there is a high concentration of non-radioactive, endogenous hormone present in the sample competing for binding sites on the antibody. This competition results in fewer radioactive hormones binding to the antibody. Therefore, the low radioactive hormone binding suggests an abundance of non-radioactive hormone in the sample.

Choice B is incorrect because low levels of radioactive hormone binding do not indicate low plasma levels of endogenous hormone. Choice C is incorrect as more antibody wouldn't affect the binding of radioactive hormone if endogenous hormone levels are high. Choice D is incorrect because the amount of radioactive hormone needed is not related to the binding observed in the assay.