Nokea
In which of the following GIT infections, the bacteria adhere to the intestine wall and produce toxins:
- A. Shigella spp.
- B. Enteropathogenic E.coli (EPEC)
- C. Salmonella spp.
- D. Vibrio cholerae
Correct Answer: B
Rationale: Rationale:
1. Enteropathogenic E.coli (EPEC) adheres to the intestinal wall using bundle-forming pili.
2. EPEC produces toxins that disrupt cell structure and function, leading to diarrhea.
3. Shigella spp. invade intestinal cells, Salmonella spp. invade and multiply within cells, and Vibrio cholerae produces cholera toxin causing profuse watery diarrhea.
Summary:
A, C, and D choices are incorrect because they do not specifically adhere to the intestine wall and produce toxins like EPEC does.
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Legionella pneumophila is:
- A. Gram-negative coccus
- B. Gram-negative spiral bacterium
- C. Gram-negative coccobacillus
- D. Gram-positive coccobacillus
Correct Answer: C
Rationale: Legionella pneumophila is a Gram-negative coccobacillus. It is not a coccus (round shape), spiral bacterium, or Gram-positive coccobacillus. The correct answer is C because Legionella pneumophila has a characteristic coccobacillary shape and stains Gram-negative due to its cell wall composition. The other choices are incorrect because they do not accurately describe the morphology or staining characteristics of Legionella pneumophila.
Cytoplasmic membrane of the prokaryotes consists of:
- A. Phospholipids
- B. Proteins
- C. Carbohydrates
- D. All of the above
Correct Answer: D
Rationale: The correct answer is D: All of the above. The cytoplasmic membrane of prokaryotes consists of phospholipids which form a lipid bilayer, providing structure and selective permeability. Proteins are embedded in this membrane, serving various functions like transport and signaling. Carbohydrates may also be present on the surface for cell recognition. Therefore, all three components (phospholipids, proteins, and carbohydrates) are essential parts of the prokaryotic cytoplasmic membrane. Option A, B, and C individually do not fully encapsulate the complexity and composition of the prokaryotic cytoplasmic membrane.
Axial filaments consist of
- A. proteins
- B. polyphosphates
- C. volutin granules
- D. carbohydrates
Correct Answer: A
Rationale: Axial filaments, also known as endoflagella, are composed of proteins. These proteins form a helical structure that enables certain bacteria, such as spirochetes, to move in a corkscrew-like motion. Proteins are the main structural component of axial filaments due to their flexibility and ability to generate movement. Choice B, polyphosphates, are not typically associated with axial filaments, as they are more involved in energy storage and transfer. Choice C, volutin granules, are storage inclusions of inorganic polyphosphate and are not part of axial filaments. Choice D, carbohydrates, are not the primary component of axial filaments which are protein-based structures responsible for bacterial motility.
Which of the following media is often used to grow Mycobacterium tuberculosis?
- A. Middlebrook’s medium
- B. Heart infusion broth
- C. MacConkey agar
- D. Middlebrook’s medium
Correct Answer: D
Rationale: The correct answer is D: Middlebrook's medium. This medium is specifically designed for the growth of Mycobacterium tuberculosis due to its low concentration of malachite green, which inhibits the growth of other bacteria but not Mycobacterium species. Middlebrook's medium also contains various nutrients that support the growth of Mycobacterium tuberculosis.
A: Middlebrook's medium is the correct answer, not incorrect.
B: Heart infusion broth is a general-purpose medium, not specific for Mycobacterium tuberculosis.
C: MacConkey agar is selective for gram-negative bacteria and would not support the growth of Mycobacterium tuberculosis.
In summary, Middlebrook's medium is the ideal choice for cultivating Mycobacterium tuberculosis due to its specific formulation that promotes the growth of this pathogen while inhibiting other bacteria.
Choose which one fo the following microorganisms is an example of obligate parasitism
- A. HIV
- B. E. Coli
- C. S. Epidermidis
- D. Pseudomonas aeruginosa
Correct Answer: A
Rationale: The correct answer is A: HIV. HIV is an example of obligate parasitism because it requires a host cell to replicate and survive. It cannot live independently outside of a host organism. In contrast, choices B, C, and D are not obligate parasites. E. Coli and S. Epidermidis are examples of commensal bacteria that can live in the host without causing harm. Pseudomonas aeruginosa can be both an opportunistic pathogen and a free-living organism, not strictly dependent on a host for survival.