Nokea
Most reliable test for detection of acute hepatitis A infection is
- A. Western blot assay for IgG anti-HAV in serum
- B. ELISA test for IgM anti-HAV in serum and HAV-Ag in stools
- C. ELISA test for IgG anti-HAV in serum
- D. None of the above
Correct Answer: B
Rationale: The correct answer is B: ELISA test for IgM anti-HAV in serum and HAV-Ag in stools. This test is the most reliable for detecting acute hepatitis A infection because IgM antibodies are produced early in the infection, indicating an active infection. Detection of HAV antigen in stools also confirms active viral shedding.
A: Western blot assay for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
C: ELISA test for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
D: None of the above is incorrect as option B is the most appropriate choice for detecting acute hepatitis A infection.
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A child is presumably ill with diphtheria. A specimen of affected mucous membrane of his pharynx was taken for analysis. The smear was stained and microscopic examination revealed yellow rods with dark blue thickenings on their ends. What structural element of a germ cell was revealed in the detected microorganisms?
- A. Volutin granules
- B. Plasmids
- C. Capsule
- D. Spores
Correct Answer: A
Rationale: The correct answer is A: Volutin granules. Volutin granules are metachromatic granules that stain dark blue with methylene blue or Leishman stain. In diphtheria-causing bacteria, Corynebacterium diphtheriae, these granules are typically seen as yellow rods with dark blue thickenings on their ends when stained. Volutin granules are storage inclusions composed of polymetaphosphate that serve as an energy reserve for the bacteria. The presence of these granules in the detected microorganisms indicates the characteristic feature of Corynebacterium diphtheriae.
Summary of other choices:
B: Plasmids are extrachromosomal DNA molecules that are not typically visible on stained smears.
C: Capsules are outer protective layers that are not visible on stained smears.
D: Spores are reproductive structures formed by certain bacteria, not typically seen in Corynebacterium diphtheriae.
In 8 days after a surgery the patient develops tetatus. The surgeon suspects this condition to be caused by suture material contaminated by tetanus agent. The material is delivered to a bacteriological laboratory. What nutrient medium is required for primary inoculation of the suture material?
- A. Sabouraud agar
- B. Endo agar
- C. Hiss medium
- D. Kitt-Tarozzi medium
Correct Answer: D
Rationale: The correct answer is D, Kitt-Tarozzi medium. This medium is specifically designed for the isolation and cultivation of Clostridium tetani, the bacteria responsible for tetanus. The other options, Sabouraud agar, Endo agar, and Hiss medium, are not suitable for the growth of Clostridium tetani. Sabouraud agar is used for fungal cultures, Endo agar is a selective medium for enteric bacteria, and Hiss medium is used for the cultivation of anaerobic bacteria other than Clostridium tetani. Therefore, the Kitt-Tarozzi medium is the most appropriate choice for isolating and identifying the tetanus agent in this scenario.
The term “infectious process†means “all biological processes carried out after…â€
- A. The attachment and penetration of microbes into the microorganism
- B. The attachment and penetration of microbes into the microorganisms regardless of whether or not there is clinical manifestation
- C. The penetration of microbes into the macroorganism and always manifest clinically
- D. No correct answer
Correct Answer: B
Rationale: The correct answer is B because the term "infectious process" refers to all biological processes that occur after the attachment and penetration of microbes into the host organism, regardless of whether there are clinical manifestations. This includes the colonization, multiplication, and spread of the microorganisms within the host.
Explanation:
A: Incorrect because it only mentions the attachment and penetration of microbes into the microorganism, missing the subsequent processes.
C: Incorrect because it is too specific, stating that the penetration of microbes always manifests clinically, which is not always the case.
D: Incorrect because there is a correct answer, which is B, as explained above.
Bacterial endotoxins are:
- A. proteins in the cell wall
- B. secreted into the environment
- C. components of the gram-positive cell wall
- D. components of the gram-negative cell wall
Correct Answer: D
Rationale: The correct answer is D: components of the gram-negative cell wall. Bacterial endotoxins are lipopolysaccharides found in the outer membrane of gram-negative bacteria. They are released upon bacterial cell lysis and can cause severe immune responses.
A: Proteins in the cell wall - This is incorrect because endotoxins are not proteins but rather lipopolysaccharides.
B: Secreted into the environment - Endotoxins are not actively secreted but are released upon cell death or lysis.
C: Components of the gram-positive cell wall - Gram-positive bacteria do not have an outer membrane with lipopolysaccharides, so endotoxins are not found in the gram-positive cell wall.
Microscopy of sputum revealed acid-fast bacilli stained red against a blue background using Ziehl-Neelsen staining. What disease does this finding suggest?
- A. Tuberculosis
- B. Diphtheria
- C. Pneumonia
- D. Leprosy
Correct Answer: A
Rationale: The presence of acid-fast bacilli stained red against a blue background indicates mycobacteria, specifically Mycobacterium tuberculosis in this case. Ziehl-Neelsen staining is commonly used to detect tuberculosis. Diphtheria is caused by Corynebacterium diphtheriae, which does not stain with Ziehl-Neelsen. Pneumonia is a general term for lung infections caused by various pathogens. Leprosy is caused by Mycobacterium leprae, which requires special staining methods different from Ziehl-Neelsen.