mcgraw hill microbiology test bank

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Which one of the methods is used to identify cytoplasmic inclusions?

  • A. Neisser staining
  • B. Clett staining
  • C. Ziehl-Nielsen staining
  • D. Gram staining
Correct Answer: A

Rationale: The correct answer is A: Neisser staining. This method is specifically used to identify cytoplasmic inclusions by staining them with a specific dye that highlights these structures. Neisser staining is designed to selectively target and visualize cytoplasmic inclusions, making it the most appropriate choice for this purpose.

Option B: Clett staining, Option C: Ziehl-Nielsen staining, and Option D: Gram staining are not typically used for identifying cytoplasmic inclusions. Clett staining is not a commonly known staining method for this purpose. Ziehl-Nielsen staining is primarily used to detect acid-fast bacteria like Mycobacterium tuberculosis. Gram staining is used to differentiate bacteria into Gram-positive and Gram-negative based on cell wall characteristics. Therefore, these options are not suitable for identifying cytoplasmic inclusions.

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In order to accurately identify etiology of peptic ulcer disease, polymerase chain reaction (PCR) analysis was performed on gastric biopsy specimen taken from 47-year-old patient. Eventually, the test result was positive for H. pylori. Which of the following was most likely detected in PCR analysis?

  • A. Bacterial DNA
  • B. Bacterial toxin
  • C. H. pylori bacterium itself
  • D. Bacterial enzymes – urease and catalase
Correct Answer: A

Rationale: The correct answer is A: Bacterial DNA. PCR analysis detects specific DNA sequences, so a positive result for H. pylori indicates the presence of the bacterial DNA in the gastric biopsy specimen. This method identifies the genetic material of the bacterium, not the bacterium itself (choice C) or its toxins (choice B). Additionally, PCR does not directly detect bacterial enzymes (choice D) but rather focuses on genetic material for accurate identification. Therefore, in this case, the detection of H. pylori in the PCR analysis points to the presence of bacterial DNA, confirming the etiology of peptic ulcer disease.

The nucleocapsid of viruses consists of

  • A. genome and envelop
  • B. genome and capsid
  • C. capsid and spikes
  • D. capsid and envelop
Correct Answer: B

Rationale: The correct answer is B because the nucleocapsid of viruses is composed of the genome (genetic material) and the capsid (protein coat). The genome contains the viral genetic information, while the capsid protects the genome and helps in viral attachment and entry into host cells.

Choice A is incorrect because the envelope is not part of the nucleocapsid but surrounds the capsid in some viruses. Choice C is incorrect as spikes are surface proteins on the viral envelope, not part of the nucleocapsid. Choice D is also incorrect because the envelope is external to the capsid and genome in many viruses.

Mycobacteria have in their cell walls:

  • A. High amount of polysaccharides
  • B. High amount of lipids
  • C. High amount of mycolic acids
  • D. High amount of proteins
Correct Answer: C

Rationale: The correct answer is C: High amount of mycolic acids. Mycobacteria are characterized by their unique cell wall composition, which includes a high amount of mycolic acids. Mycolic acids provide the bacteria with resistance to environmental stresses and are crucial for their pathogenicity. Polysaccharides (A) are not a prominent component of mycobacterial cell walls. While lipids (B) are present in mycobacterial cell walls, mycolic acids are the predominant lipid. Proteins (D) are also present but are not the defining feature of mycobacterial cell walls. Therefore, the correct answer is C due to the significant role mycolic acids play in the structure and function of mycobacterial cell walls.

What is the function of bacterial flagella?

  • A. Protein synthesis
  • B. Motility
  • C. Adhesion to surfaces
  • D. DNA replication
Correct Answer: B

Rationale: The correct answer is B: Motility. Bacterial flagella function in providing motility to bacteria, allowing them to move towards nutrients or away from harmful substances. Flagella are long, whip-like appendages that rotate to propel the bacteria through their environment.
Incorrect choices:
A: Protein synthesis - Bacterial flagella are not involved in protein synthesis.
C: Adhesion to surfaces - While some bacteria use flagella for attachment, their main function is not adhesion.
D: DNA replication - Flagella do not play a role in DNA replication, which occurs in the bacterial cell's nucleus.

Viral capsomeres are composed of subunits called

  • A. envelopes.
  • B. protomeres.
  • C. capsids.
  • D. nucleoids.
Correct Answer: B

Rationale: The correct answer is B: protomeres. Viral capsomeres are the building blocks of viral capsids, which are protein coats that enclose and protect the viral genetic material. These capsomeres are made up of smaller subunits called protomeres. Envelopes (A) refer to a lipid membrane surrounding some viruses, not the subunits of capsomeres. Capsids (C) are the protein coats that capsomeres make up, not the subunits themselves. Nucleoids (D) are found in bacterial cells and refer to the region where the genetic material is located, not the subunits of viral capsomeres.

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