N. Meningitidis grows on
- A. Levine agar
- B. Lowenstein-Jensen agar
- C. TCBS agar
- D. Chocolate agar
Correct Answer: D
Rationale: The correct answer is D: Chocolate agar. Neisseria meningitidis is a fastidious organism that requires enriched media for growth. Chocolate agar contains heat-treated blood that provides essential nutrients for the growth of N. meningitidis. The other choices, A: Levine agar, B: Lowenstein-Jensen agar, and C: TCBS agar, do not provide the specific nutrients required for the growth of N. meningitidis. Therefore, only choice D, Chocolate agar, supports the growth of N. meningitidis due to its enriched composition.
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A woman delivered a dead child with multiple developmental defects. What protozoan disease might have caused the intrauterine death?
- A. Toxoplasmosis
- B. Leishmaniasis
- C. Malaria
- D. Amebiasis
Correct Answer: A
Rationale: The correct answer is A: Toxoplasmosis. Toxoplasmosis is a parasitic infection caused by Toxoplasma gondii, which can be transmitted from mother to fetus during pregnancy, leading to congenital toxoplasmosis. This infection can result in stillbirth, multiple developmental defects, and intrauterine death. Leishmaniasis (B), Malaria (C), and Amebiasis (D) are caused by different parasites and do not typically lead to intrauterine death or developmental defects in the fetus.
Which of the following microorganisms can be cultured only in the footpads of mice or a species of armadillo?
- A. Mycobacterium tuberculosis
- B. Mycobacterium leprae
- C. Neisseria gonorrhoeae
- D. Mycoplasma
Correct Answer: B
Rationale: The correct answer is B: Mycobacterium leprae. This microorganism can only be cultured in the footpads of mice or a species of armadillo due to its specific growth requirements. Mycobacterium tuberculosis (choice A) can be cultured in various culture media in the laboratory. Neisseria gonorrhoeae (choice C) can be cultured on selective media like Thayer-Martin agar. Mycoplasma (choice D) can be cultured in cell cultures or specialized media. Therefore, the unique growth pattern of Mycobacterium leprae makes it the correct answer.
Sterilization is a process that:
- A. Destroys most microorganism, but spores remain viable
- B. Leads to complete destruction of all microorganisms, including resistant forms such as spores
- C. Reduces the number of microorganisms on the surface of the skin
- D. None of the above
Correct Answer: B
Rationale: The correct answer is B because sterilization is the process of completely destroying all microorganisms, including resistant forms like spores, ensuring no viable organisms remain. This is crucial for ensuring complete elimination of potential pathogens. Choice A is incorrect because sterilization should eliminate all microorganisms, including spores. Choice C is incorrect as it describes disinfection, not sterilization. Choice D is incorrect because sterilization aims to eradicate all microorganisms, not leave any viable ones behind.
A patient with a deep tissue infection had Gram-negative rods isolated from the wound culture. The bacteria were oxidase-positive and non-lactose fermenters. What is the most likely causative agent?
- A. Pseudomonas aeruginosa
- B. Escherichia coli
- C. Klebsiella pneumoniae
- D. Shigella dysenteriae
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. This is because Pseudomonas is a Gram-negative rod, oxidase-positive, and non-lactose fermenter. These characteristics are typical of Pseudomonas species.
B: Escherichia coli is a Gram-negative rod but is lactose fermenter and usually oxidase-negative.
C: Klebsiella pneumoniae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative.
D: Shigella dysenteriae is a Gram-negative rod, non-lactose fermenter, but is usually oxidase-negative and is more associated with causing shigellosis rather than deep tissue infections.
The most important virulence factor of Neisseria meningitidis is:
- A. Exotoxin
- B. Polysaccharide capsule
- C. Beta-lactamase
- D. Pyrogenic toxin
Correct Answer: B
Rationale: The correct answer is B: Polysaccharide capsule. The capsule of Neisseria meningitidis is crucial for its virulence as it helps the bacteria evade the host immune system by inhibiting phagocytosis. This allows the bacteria to survive and multiply in the host, leading to invasive infections. Exotoxins, beta-lactamase, and pyrogenic toxins are not the main virulence factors of Neisseria meningitidis and do not play as significant a role in its pathogenicity as the polysaccharide capsule.