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Which bacteria are known for causing food poisoning due to their production of enterotoxins?
- A. Clostridium botulinum
- B. Salmonella enterica
- C. Staphylococcus aureus
- D. All of the above
Correct Answer: D
Rationale: The correct answer is D, "All of the above." Clostridium botulinum produces botulinum toxin causing botulism. Salmonella enterica can produce enterotoxins causing food poisoning. Staphylococcus aureus also produces enterotoxins leading to food poisoning. Therefore, all three options can cause food poisoning due to their production of enterotoxins.
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Microscopic examination of dental plaque revealed unicellular organisms with two layers in their cytoplasm and wide pseudopodia. What is the likely causative agent?
- A. Entamoeba gingivalis
- B. Entamoeba histolytica
- C. Trichomonas tenax
- D. Lamblia intestinalis
Correct Answer: A
Rationale: Rationale:
1. Unicellular organisms with two layers in cytoplasm = Entamoeba gingivalis.
2. Wide pseudopodia = characteristic of Entamoeba genus.
3. Entamoeba histolytica causes amoebic dysentery, not dental plaque.
4. Trichomonas tenax causes periodontal disease, not dental plaque.
5. Lamblia intestinalis causes giardiasis, not related to dental plaque.
Bacteria that grow best in high temperatures are called:
- A. Mesophiles
- B. Psychrophiles
- C. Thermophiles
- D. Halophiles
Correct Answer: C
Rationale: The correct answer is C: Thermophiles. Thermophiles are bacteria that thrive in high temperatures, typically between 45-80 degrees Celsius. They have adapted to extreme heat environments and have enzymes that function optimally at high temperatures. Mesophiles (A) grow best at moderate temperatures, Psychrophiles (B) thrive in cold temperatures, and Halophiles (D) thrive in high salt concentrations. Therefore, only Thermophiles are specifically adapted to high temperatures, making it the correct choice.
Which of the following bacteria is capable of forming spores to survive in extreme conditions?
- A. Staphylococcus aureus
- B. Clostridium botulinum
- C. Escherichia coli
- D. Neisseria gonorrhoeae
Correct Answer: B
Rationale: The correct answer is B: Clostridium botulinum. This bacterium is capable of forming spores as a survival mechanism in harsh conditions. Spore formation allows it to remain dormant until conditions become favorable for growth again. Staphylococcus aureus (A), Escherichia coli (C), and Neisseria gonorrhoeae (D) do not form spores and rely on other mechanisms for survival. Staphylococcus aureus is a common skin bacterium, Escherichia coli is a gut bacterium, and Neisseria gonorrhoeae is a sexually transmitted bacterium.
The typical site for replication of DNA viruses is:
- A. Nucleus
- B. Cytoplasm
- C. Endoplasmic Reticulum
- D. Golgi Apparatus
Correct Answer: A
Rationale: The correct answer is A: Nucleus. DNA viruses replicate in the host cell's nucleus because they require access to the host cell's DNA replication machinery. The nucleus contains the necessary enzymes and factors for DNA replication to occur accurately. The DNA viruses need to utilize these host cell components to replicate their own genetic material.
Summary of why other choices are incorrect:
B: Cytoplasm - Cytoplasm lacks the necessary enzymes and machinery for accurate DNA replication.
C: Endoplasmic Reticulum - ER is involved in protein synthesis and processing, not DNA replication.
D: Golgi Apparatus - Golgi apparatus is responsible for processing and packaging proteins, not DNA replication.
Which are the most suitable materials for isolation of polio viruses?
- A. feces, duodenal and bile content
- B. blood, sputum, and urine
- C. only feces
- D. feces, nasopharyngeal swabs, and cerebrospinal fluid
Correct Answer: D
Rationale: The correct answer is D because polio viruses are primarily found in the gastrointestinal tract and respiratory secretions. Feces contain the highest concentration of the virus, while nasopharyngeal swabs and cerebrospinal fluid can also contain the virus. Blood, sputum, and urine do not typically contain high levels of polio virus. Choice A is incorrect as bile content is not a common site for polio virus isolation. Choice C is incorrect because only testing feces may not provide a comprehensive picture of the infection.