Nokea
Which bacteria are known for producing toxins that can cause severe gastrointestinal illness?
- A. Clostridium botulinum
- B. Vibrio cholerae
- C. Escherichia coli
- D. All of the above
Correct Answer: D
Rationale: The correct answer is D, "All of the above." Clostridium botulinum produces botulinum toxin causing botulism, Vibrio cholerae produces cholera toxin leading to severe diarrhea, and certain strains of Escherichia coli produce toxins causing gastrointestinal illness. Therefore, all three bacteria are known for producing toxins that can cause severe gastrointestinal illness. Choices A, B, and C individually represent bacteria known for producing toxins that can cause severe gastrointestinal illness, making them incorrect individual choices.
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Which one of all the clostridia that are associated with the disease myonecrosis/gas-gangrene can produce enterotoxin and could be responsible for the development of food intoxications:
- A. Clostridium septicum
- B. Clostridium perfringens
- C. Clostridium histolyticum
- D. Clostridium novyi (oedemmateins)
Correct Answer: B
Rationale: The correct answer is B: Clostridium perfringens. This bacterium is associated with myonecrosis/gas gangrene and can produce enterotoxin causing food intoxication. Clostridium septicum (A) is associated with wound infections but not known for food intoxications. Clostridium histolyticum (C) is not typically associated with myonecrosis or food intoxications. Clostridium novyi (D) is known for causing black disease in sheep and cattle, not food intoxications.
A laboratory received a sample of water used in drug production for sanitary and viral analysis. What group of viruses will indicate fecal contamination of water and thus the need for its additional purification?
- A. Picornaviridae
- B. Herpesviridae
- C. Flaviviridae
- D. Retroviridae
Correct Answer: A
Rationale: The correct answer is A: Picornaviridae. Picornaviruses, such as enteroviruses, are commonly found in the feces of infected individuals. Therefore, their presence in water indicates fecal contamination and the need for additional purification.
B: Herpesviridae are not typically associated with fecal contamination.
C: Flaviviridae are mainly transmitted through arthropods like mosquitoes, not fecal contamination.
D: Retroviridae, including HIV, are not typically excreted in high levels in feces and are not indicators of fecal contamination.
Most reliable test for detection of acute hepatitis A infection is
- A. Western blot assay for IgG anti-HAV in serum
- B. ELISA test for IgM anti-HAV in serum and HAV-Ag in stools
- C. ELISA test for IgG anti-HAV in serum
- D. None of the above
Correct Answer: B
Rationale: The correct answer is B: ELISA test for IgM anti-HAV in serum and HAV-Ag in stools. This test is the most reliable for detecting acute hepatitis A infection because IgM antibodies are produced early in the infection, indicating an active infection. Detection of HAV antigen in stools also confirms active viral shedding.
A: Western blot assay for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
C: ELISA test for IgG anti-HAV in serum is not the most reliable for acute infection detection as IgG antibodies are produced later in the infection and indicate past exposure or immunity.
D: None of the above is incorrect as option B is the most appropriate choice for detecting acute hepatitis A infection.
Transcription of viral nucleic acid to mRNA is not needed in the case of:
- A. There is no correct answer
- B. Single strand DNA viruses
- C. Double strand DNA viruses
- D. Positive strand RNA viruses
Correct Answer: D
Rationale: The correct answer is D: Positive strand RNA viruses. These viruses have RNA genomes that can be directly translated by host ribosomes without the need for transcription. In contrast, single-strand DNA and double-strand DNA viruses require transcription to generate mRNA for translation. Choice A is incorrect as there is a correct answer. Choice B and C are incorrect because both single-strand DNA and double-strand DNA viruses require transcription for mRNA synthesis.
A patient with pneumonia had sputum cultured, revealing Gram-negative diplococci. The bacteria were oxidase-positive and fermentative. What is the most likely causative agent?
- A. Neisseria meningitidis
- B. Neisseria gonorrhoeae
- C. Moraxella catarrhalis
- D. Haemophilus influenzae
Correct Answer: A
Rationale: The correct answer is A: Neisseria meningitidis. This is because Neisseria meningitidis is a Gram-negative diplococcus that is oxidase-positive and fermentative. Neisseria gonorrhoeae is also a Gram-negative diplococcus, but it is not fermentative. Moraxella catarrhalis is oxidase-positive but not fermentative. Haemophilus influenzae is Gram-negative but is not a diplococcus, and it is not fermentative. Therefore, based on the characteristics provided in the question, Neisseria meningitidis is the most likely causative agent.