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Which of the following is characteristic of obligate anaerobes?
- A. They require oxygen to survive
- B. They can survive in the presence or absence of oxygen
- C. They cannot tolerate oxygen
- D. They prefer low levels of oxygen
Correct Answer: C
Rationale: The correct answer is C: Obligate anaerobes cannot tolerate oxygen. They lack the enzymes needed to detoxify reactive oxygen species generated in the presence of oxygen. This makes them unable to survive in oxygen-rich environments. Choice A is incorrect because obligate anaerobes do not require oxygen to survive. Choice B is incorrect because obligate anaerobes specifically require the absence of oxygen. Choice D is incorrect because obligate anaerobes cannot even tolerate low levels of oxygen.
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Which bacteria produces an enterotoxin that causes diarrhea?
- A. Vibrio cholerae
- B. Clostridium botulinum
- C. Escherichia coli
- D. Streptococcus pneumoniae
Correct Answer: A
Rationale: The correct answer is A: Vibrio cholerae. This bacterium produces the cholera toxin, an enterotoxin that causes profuse watery diarrhea by disrupting the normal function of the intestines. Vibrio cholerae is specifically known for causing cholera, a severe diarrheal disease. Clostridium botulinum (B) produces botulinum toxin, causing botulism (paralysis, not diarrhea). Escherichia coli (C) can produce enterotoxins, but the question specifies a specific enterotoxin causing diarrhea, which is not a characteristic of all strains of E. coli. Streptococcus pneumoniae (D) is a common cause of respiratory infections but does not produce an enterotoxin causing diarrhea.
By precipitation of tuberculin, a purified protein derivative (PPD) is obtained. It is used in:
- A. Mantoux test
- B. Schick test
- C. Dick test
- D. None of the above
Correct Answer: A
Rationale: The correct answer is A: Mantoux test. The Mantoux test is used to screen for tuberculosis by injecting PPD intradermally. The presence of a delayed hypersensitivity reaction indicates prior exposure to Mycobacterium tuberculosis. The Schick test (B) is used to test for immunity to diphtheria, while the Dick test (C) is used to test for immunity to scarlet fever. Therefore, the correct use of PPD is in the Mantoux test for tuberculosis screening.
A patient has a necrotizing phlegmon of his lower extremity. A doctor suspects a gas gangrene. Microscopy reveals grampositive bacilli. In order to confirm the diagnosis further bacteriological tests should include inoculation of the material into the following nutrient medium:
- A. Kitt-Tarozzi medium
- B. Endo agar
- C. Meat-peptone agar
- D. Levine agar
Correct Answer: A
Rationale: The correct answer is A: Kitt-Tarozzi medium. This medium is specifically designed to culture Clostridium species, the causative agents of gas gangrene. Clostridium species are gram-positive bacilli that produce gas during their metabolism. Inoculating the material into Kitt-Tarozzi medium allows for the selective growth of Clostridium species, aiding in the confirmation of a gas gangrene diagnosis.
Other choices are incorrect:
B: Endo agar is used for the isolation of gram-negative enteric bacteria.
C: Meat-peptone agar is a general-purpose medium and not specific for Clostridium species.
D: Levine agar is used for the isolation of Salmonella and Shigella species, not Clostridium.
Warts are commonly caused by the:
- A. human papillomavirus
- B. herpesvirus
- C. HIV
- D. varicella-zoster virus
Correct Answer: A
Rationale: Step-by-step rationale:
1. Warts are caused by human papillomavirus (HPV) due to its ability to infect the skin.
2. HPV infects the top layer of skin and causes the rapid growth of cells, leading to the formation of warts.
3. Herpesvirus (choice B) causes herpes, not warts.
4. HIV (choice C) weakens the immune system but does not directly cause warts.
5. Varicella-zoster virus (choice D) causes chickenpox and shingles, not warts.
Summary:
Choice A is correct as warts are primarily caused by HPV, which infects the skin cells. Choices B, C, and D are incorrect as they are associated with different viral infections that do not lead to the development of warts.
Typhoid fever is caused by the bacterium:
- A. Escherichia coli
- B. Salmonella enterica
- C. Shigella flexneri
- D. Staphylococcus aureus
Correct Answer: B
Rationale: The correct answer is B: Salmonella enterica. Typhoid fever is caused by the bacterium Salmonella enterica serotype Typhi. This specific strain of Salmonella is responsible for typhoid fever, a serious illness characterized by high fever, abdominal pain, and diarrhea. Escherichia coli (choice A) is not the causative agent of typhoid fever. Shigella flexneri (choice C) causes bacillary dysentery, not typhoid fever. Staphylococcus aureus (choice D) can cause food poisoning but is not associated with typhoid fever.