mastering microbiology test bank

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Which of the following is used to test for bacterial antibiotic susceptibility?

  • A. Gram stain
  • B. Catalase test
  • C. Kirby-Bauer disk diffusion test
  • D. Endospore stain
Correct Answer: C

Rationale: The correct answer is C: Kirby-Bauer disk diffusion test. This test is used to determine the antibiotic susceptibility of bacteria by measuring the zone of inhibition around antibiotic-soaked paper disks. It helps identify which antibiotics are effective against the bacteria being tested. This test is crucial in guiding antibiotic treatment decisions.

A: Gram stain is used to classify bacteria based on their cell wall composition and morphology, not for antibiotic susceptibility testing.
B: Catalase test is used to differentiate between catalase-positive and catalase-negative bacteria, not for antibiotic susceptibility testing.
D: Endospore stain is used to detect the presence of endospores in bacteria, not for antibiotic susceptibility testing.

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Which of the following bacteria is a common cause of pneumonia?

  • A. Streptococcus pneumoniae
  • B. Mycobacterium tuberculosis
  • C. Pseudomonas aeruginosa
  • D. All of the above
Correct Answer: D

Rationale: The correct answer is D: All of the above.
- Streptococcus pneumoniae is a common cause of community-acquired pneumonia.
- Mycobacterium tuberculosis can cause pulmonary tuberculosis, which can present as pneumonia-like symptoms.
- Pseudomonas aeruginosa is an opportunistic pathogen that can cause pneumonia in immunocompromised individuals.
Therefore, all three bacteria can cause pneumonia, making option D the correct choice. Options A, B, and C are incorrect because they each represent a bacterium that can cause pneumonia, so only selecting one of them would not encompass all common causes of pneumonia.

Poxviruses are the largest viruses, almost visible on a light microscope.

  • A. TRUE
  • B. FALSE
  • C. Not Sure
  • D. Partially
Correct Answer: A

Rationale: The correct answer is A: TRUE. Poxviruses are indeed the largest viruses, ranging from 200-450 nm in size, which is close to the resolution limit of a light microscope. This makes them almost visible under a light microscope. The other choices are incorrect because poxviruses are not smaller or uncertain in size (choices B and C), nor are they partially visible - they are almost visible due to their relatively large size.

Which of the following bacteria are unable to synthesize ATP?

  • A. Chlamydia
  • B. Enterococci
  • C. Rickettsia
  • D. Staphylococci
Correct Answer: A

Rationale: The correct answer is A: Chlamydia. Chlamydia lacks the essential enzymes for ATP synthesis and relies on host cell ATP. Enterococci, Rickettsia, and Staphylococci possess the necessary enzymes for ATP synthesis through cellular respiration. Chlamydia's inability to synthesize ATP makes it dependent on the host cell for energy production, distinguishing it from the other bacteria listed.

Warts are commonly caused by the:

  • A. human papillomavirus
  • B. herpesvirus
  • C. HIV
  • D. varicella-zoster virus
Correct Answer: A

Rationale: Step-by-step rationale:
1. Warts are caused by human papillomavirus (HPV) due to its ability to infect the skin.
2. HPV infects the top layer of skin and causes the rapid growth of cells, leading to the formation of warts.
3. Herpesvirus (choice B) causes herpes, not warts.
4. HIV (choice C) weakens the immune system but does not directly cause warts.
5. Varicella-zoster virus (choice D) causes chickenpox and shingles, not warts.

Summary:
Choice A is correct as warts are primarily caused by HPV, which infects the skin cells. Choices B, C, and D are incorrect as they are associated with different viral infections that do not lead to the development of warts.

The Gram stain is used to differentiate bacteria based on:

  • A. Cell shape
  • B. Cell wall structure
  • C. Flagella composition
  • D. Nutrient requirements
Correct Answer: B

Rationale: The Gram stain differentiates bacteria based on cell wall structure. It classifies bacteria into Gram-positive (purple) and Gram-negative (pink) based on the presence of peptidoglycan in the cell wall. This method helps in identifying the bacterial species and determining appropriate treatment. Option A (Cell shape) is incorrect because the Gram stain focuses on cell wall composition, not cell shape. Option C (Flagella composition) is incorrect as the Gram stain does not assess flagella. Option D (Nutrient requirements) is incorrect as it does not relate to the purpose of the Gram stain.

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