Nokea
You have suffered a needle stick injury after giving a patient an IM injection,but you have no information about the patient’s HIV status. What is the most appropriate method for obtaining this information about the patient?
- A. You should ask the patient to authorize HIV testing as soon as possible.
- B. The nurse manager for the unit is responsible for obtaining the information.
- C. The occupational health nurse should discuss HIV status with the patient.
- D. HIV testing should be done the next time blood is drawn for other tests.
Correct Answer: C
Rationale: The occupational health nurse is trained to handle situations involving potential exposure to bloodborne pathogens. They can appropriately discuss the need for HIV testing with the patient and ensure that the process is handled confidentially and professionally. The nurse manager and the injured nurse should not directly approach the patient about this sensitive issue.
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Which of the following cells are 'professional' antigen presenting cells
- A. Neutrophils, dendritic cells, B cells
- B. B cells, T cells, dendritic cells
- C. Macrophages, B cells, dendritic cells
- D. Neutrophils, epithelial cells, dendritic cells
Correct Answer: C
Rationale: The correct answer is C: Macrophages, B cells, dendritic cells. Professional antigen-presenting cells are specialized immune cells that effectively present antigens to T cells. Macrophages, B cells, and dendritic cells are considered professional APCs because they express major histocompatibility complex (MHC) molecules and co-stimulatory molecules, allowing them to effectively activate T cells. Neutrophils and epithelial cells are not considered professional APCs as they have limited ability to present antigens to T cells. B cells and T cells are not APCs themselves, but B cells can act as APCs when they present antigens to T cells in the context of an immune response.
Which of the following are not found in Cyanobacteria?
- A. thylakoids
- B. gas vesicles
- C. chloroplasts
- D. heterocysts
Correct Answer: C
Rationale: The correct answer is C: chloroplasts. Cyanobacteria do not have chloroplasts because they are prokaryotic organisms and do not possess membrane-bound organelles like chloroplasts found in eukaryotic cells. Instead, they perform photosynthesis using thylakoids, which are membrane-bound compartments where photosynthesis occurs. Gas vesicles are also present in cyanobacteria and help regulate their buoyancy. Heterocysts are specialized cells that aid in nitrogen fixation, a process that allows cyanobacteria to convert atmospheric nitrogen into a form that can be used by the organism. Therefore, the absence of chloroplasts in cyanobacteria makes choice C the correct answer.
Which enzyme catalyzes the following reaction? Gap 5' - G G-A-T-C-C—3' ⋮ ⋮ ⋮ ⋮ ⋮ 3' - C-C-T-A-G G—5' Gap 5' - G-G-A-T-C-C—3' ⋮ ⋮ ⋮ ⋮ ⋮ 3' - C-C-T-A-G-G—5'
- A. DNA ligase
- B. DNA endonuclease
- C. None of the options are correct
- D. DNA polymerase 1
Correct Answer: A
Rationale: The correct answer is A: DNA ligase. DNA ligase catalyzes the joining of two DNA strands by forming a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another. In the given reaction, the enzyme is needed to seal the nick in the DNA strands after the Okazaki fragments have been synthesized during DNA replication.
Explanation of other choices:
B: DNA endonuclease - Endonucleases cleave the phosphodiester bond within a polynucleotide chain, not join two DNA strands.
C: None of the options are correct - This is incorrect as DNA ligase is the correct enzyme for this reaction.
D: DNA polymerase 1 - DNA polymerase synthesizes new DNA strands by adding nucleotides to the 3' end of the growing chain, not joining two DNA strands.
Who introduced the concept of discrimination between self and non-self?
- A. Elie Metchnikoff
- B. Louis Pasteur
- C. Paul Ehrlich
- D. Charles Janeway
Correct Answer: C
Rationale: Paul Ehrlich introduced the concept of discrimination between self and non-self through his "horror autotoxicus" theory. He proposed that the immune system can differentiate between harmful non-self antigens and self-antigens. Ehrlich's theory laid the foundation for understanding immune responses and the development of immunology. Metchnikoff focused on phagocytosis, Pasteur on microbiology, and Janeway on innate immunity, not specifically on self vs. non-self discrimination.
Which disease is an example of Type V hypersensitivity?
- A. Multiple sclerosis
- B. Grave's disease
- C. Rheumatoid arthritis
- D. Type 1 diabetes mellitus
Correct Answer: B
Rationale: The correct answer is B, Grave's disease, for Type V hypersensitivity. In Type V hypersensitivity, antibodies target cell surface receptors, leading to abnormal stimulation or blockage. In Grave's disease, antibodies target the thyroid-stimulating hormone receptor, leading to excessive thyroid hormone production. Multiple sclerosis (A) is an example of Type IV hypersensitivity involving T cell-mediated response. Rheumatoid arthritis (C) is an example of Type III hypersensitivity involving immune complex deposition. Type 1 diabetes mellitus (D) is an example of Type II hypersensitivity involving antibody-mediated destruction of cells.