Nokea
A smear of sputum from the patient with suspected lobar pneumonia was stained with the use of the following stains and reagents: solution of gentian violet, Lugol’s solution, 960 alcohol, watermagenta. What staining method was applied in this case?
Correct Answer: A
Rationale: The correct answer is A: Gram staining method. The rationale is as follows:
1. Gentian violet is a primary stain used in the Gram staining procedure to stain the bacterial cells.
2. Lugol's solution is a mordant that helps to fix the crystal violet stain in Gram-positive bacteria.
3. 95% alcohol is used as a decolorizing agent in the Gram staining process to remove the stain from Gram-negative bacteria.
4. Watermagenta is not a standard reagent in the Gram staining method.
Therefore, based on the reagents mentioned, the staining method applied in this case is the Gram staining method.
Other choices are incorrect:
B: Ziehl-Nielsen's - This staining method is used for acid-fast staining, not for lobar pneumonia.
C: Romanovsky - This staining method is used for blood smears, not for bacterial staining in pneumonia.
D: Neisser - This staining method is not commonly used in routine bacterial staining procedures.