Nokea
A stool sample from a patient with persistent diarrhea revealed cysts with four nuclei. What is the most likely causative agent?
- A. Entamoeba histolytica
- B. Balantidium coli
- C. Giardia lamblia
- D. Trichomonas hominis
Correct Answer: A
Rationale: The correct answer is A: Entamoeba histolytica. This is because it is the only choice that matches the description of cysts with four nuclei, characteristic of Entamoeba histolytica. Entamoeba histolytica is a protozoan parasite that causes amoebiasis, leading to symptoms like persistent diarrhea.
The other choices can be eliminated for the following reasons:
B: Balantidium coli - It is a ciliated protozoan that causes dysentery in humans but does not produce cysts with four nuclei.
C: Giardia lamblia - It is a flagellated protozoan that causes giardiasis, but it forms cysts with only two nuclei.
D: Trichomonas hominis - It is a flagellated protozoan found in the human intestine, but it does not produce cysts with four nuclei.
Therefore, based on the description of cysts with four nuclei in the stool sample,
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Specify the microbiological characteristic of the air:
- A. Contaminated
- B. Within the permissible limits
- C. Almost pure
- D. Pure
Correct Answer: A
Rationale: The correct answer is A: Contaminated. The air is never completely free of microorganisms, so it is considered contaminated. Microorganisms are ubiquitous in the environment. Permissible limits (B) imply a specific standard, which is not relevant to the general microbiological characteristic of air. Almost pure (C) and pure (D) are not accurate as air always contains some level of microorganisms.
Dwellers of a village noticed mass mortality of rats in some farms. It was suspected that the animals might have died from plague. What postmortal analyses should be conduced in order to establish the causative agent of the infection as soon as possible?
- A. Ring precipitation reaction
- B. Agglutination reaction
- C. Passive agglutination reaction
- D. Neutralization reaction
Correct Answer: B
Rationale: The correct answer is B: Agglutination reaction. This test is commonly used to detect specific antibodies or antigens. In this case, it can be used to detect plague antigens in the rat tissues or antibodies in the villagers. This test is rapid, sensitive, and can help establish the presence of the causative agent quickly.
A: Ring precipitation reaction is not typically used for diagnosing plague and is more commonly used in other types of infections.
C: Passive agglutination reaction is not specific for plague and is more commonly used for detecting specific antibodies in diseases like syphilis.
D: Neutralization reaction is not typically used for diagnosing plague and is more commonly used to determine the presence of neutralizing antibodies in viral infections.
During bacteriological examination of sputum of a child with choking cough and fever there were revealed glossy smooth colonies growing on casein-charcoal agar and reminding of mercury drops. Microscopic examination revealed short Gram-negative bacteria. What microorganism was secured from the sputum?
- A. Bordetella pertussis
- B. Haemophylus influenzae
- C. Corynebacterium dyphtheriae
- D. Klebsiella pneumoniae
Correct Answer: A
Rationale: The correct answer is A: Bordetella pertussis. Glossy smooth colonies growing on casein-charcoal agar resembling mercury drops indicate Bordetella pertussis, the causative agent of whooping cough. The presence of short Gram-negative bacteria further supports this identification. Option B, Haemophilus influenzae, typically grows as small, round, translucent colonies on chocolate agar. Option C, Corynebacterium diphtheriae, forms gray colonies on tellurite agar. Option D, Klebsiella pneumoniae, appears as mucoid colonies on MacConkey agar.
A patient was admitted to the hospital on the 7th day of the disease with complaints of high temperature, headache, pain in the muscles, especially in calf muscles. Dermal integuments and scleras are icteric. There is hemorrhagic rash on the skin. Urine is bloody. The patient was fishing two weeks ago. What is the most likely diagnosis?
- A. Leptospirosis
- B. Yersiniosis
- C. Salmonellosis
- D. Brucellosis
Correct Answer: A
Rationale: The most likely diagnosis is A: Leptospirosis. Leptospirosis is caused by the spirochete Leptospira interrogans and is commonly transmitted through contact with water contaminated by animal urine, such as in this case of fishing. The symptoms described match those of leptospirosis, including high fever, headache, muscle pain, jaundice, hemorrhagic rash, and bloody urine. The calf muscle pain is a characteristic symptom of leptospirosis known as "Weil's disease." Yersiniosis (B) typically presents with abdominal symptoms, Salmonellosis (C) with gastrointestinal symptoms, and Brucellosis (D) with flu-like symptoms. Therefore, based on the symptoms and history provided, Leptospirosis is the most likely diagnosis.
Researchers of a bacteriological laboratory examine tinned meat for botulinic toxin. For this purpose a group of mice was injected with an extract of the material under examination and antitoxic antibotulinic serum of A, B, E types. A control group of mice was injected with the same extract but without antibotulinic serum. What serological reaction was applied?
- A. Neutralization
- B. Precipitation
- C. Complement binding
- D. Double immune diffusion
Correct Answer: A
Rationale: The correct answer is A: Neutralization. In this experiment, the researchers used antitoxic antibotulinic serum of different types to neutralize the botulinic toxin in tinned meat extract injected into mice. Neutralization is the process of rendering a toxin harmless by combining it with specific antibodies. The purpose of injecting the antitoxic serum was to neutralize the toxin and prevent it from causing harm to the mice. This serological reaction of neutralization directly aligns with the objective of the experiment.
Summary of other choices:
B: Precipitation - Precipitation involves the formation of a visible insoluble complex when an antigen reacts with its corresponding antibody. This process was not described in the scenario.
C: Complement binding - Complement binding involves the activation of the complement system by antigen-antibody complexes. This was not the main focus of the experiment described.
D: Double immune diffusion - This technique is used to detect the presence of specific antigens or antibodies