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Bacteriology testing is one of the methods for laboratory diagnostics of diphtheria. To grow the
- A. diphtheriae, it is necessary to know the proper conditions for causative agent cultivation. What nutrient media are optimal for Corynebacterium diphtheriae cultivation?
- B. Sugar meat pepton broth, sugar meat pepton agar
- C. Salt egg yolk agar
- D. Endomedium, Ploskirev medium
Correct Answer: B
Rationale: The correct answer is B: Sugar meat pepton broth, sugar meat pepton agar. These nutrient media are optimal for Corynebacterium diphtheriae cultivation due to their ability to support the growth of this bacterium. Sugar meat pepton broth provides a liquid medium with essential nutrients, while sugar meat pepton agar provides a solid surface for bacterial growth and isolation.
Incorrect choices:
A: Diphtheriae does not require specific conditions for cultivation.
C: Salt egg yolk agar is not commonly used for Corynebacterium diphtheriae.
D: Endomedium and Ploskirev medium are not standard media for diphtheria testing.
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Antibiotics are distributed into following groups:
- A. Blockaders of protein synthesis
- B. Blockaders of cell wall synthesis
- C. Alternators of bacterial cytoplasm
- D. Nothing from above
Correct Answer: A
Rationale: The correct answer is A: Blockaders of protein synthesis because antibiotics in this group target bacterial ribosomes, preventing protein synthesis. This disrupts bacterial growth and replication. Choice B is incorrect as cell wall synthesis inhibitors target a different bacterial component. Choice C is incorrect as antibiotics do not alter bacterial cytoplasm. Choice D is incorrect as antibiotics do fall into different categories based on their mechanism of action.
Which of the following bacteria is known for causing the disease known as whooping cough?
- A. Bordetella pertussis
- B. Clostridium tetani
- C. Haemophilus influenzae
- D. Staphylococcus aureus
Correct Answer: A
Rationale: The correct answer is A: Bordetella pertussis. This bacterium is known for causing whooping cough due to its ability to produce toxins that damage the respiratory tract, leading to severe coughing fits. Clostridium tetani causes tetanus, Haemophilus influenzae can cause respiratory infections but not whooping cough, and Staphylococcus aureus is more commonly associated with skin infections. Therefore, A is the correct choice based on its specific association with the disease in question.
Listeria monocytogenes are:
- A. Gram-negative rod-shaped bacteria
- B. Gram-positive spherical bacteria
- C. Gram-positive rod-shaped bacteria
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C: Gram-positive rod-shaped bacteria. Listeria monocytogenes is a Gram-positive bacterium, as it retains the crystal violet stain during the Gram staining process. The bacteria are rod-shaped, not spherical. Choice A is incorrect as Listeria monocytogenes is not Gram-negative. Choice D is incorrect as Listeria monocytogenes does fall under the category of Gram-positive bacteria. The key is to remember the characteristics of Listeria monocytogenes, which are Gram-positive and rod-shaped.
Which of the following proteins are encoded by herpesviruses and required for viral DNA replication?
- A. viral DNA polymerase
- B. ribonucleotide reductase
- C. neuraminidase
- D. thymidine kinase
Correct Answer: A
Rationale: The correct answer is A: viral DNA polymerase. This enzyme is essential for replicating the viral DNA during the herpesvirus life cycle. It catalyzes the synthesis of new DNA strands using the viral genomic template. Ribonucleotide reductase (B) is involved in nucleotide synthesis, but not directly in DNA replication. Neuraminidase (C) is an enzyme found in influenza viruses, not herpesviruses. Thymidine kinase (D) is an enzyme that phosphorylates thymidine, but its role is more related to nucleotide metabolism rather than viral DNA replication.
A child with diarrhea had stool microscopy revealing cysts with four nuclei. What is the most likely causative agent?
- A. Entamoeba histolytica
- B. Giardia lamblia
- C. Balantidium coli
- D. Trichomonas hominis
Correct Answer: A
Rationale: The correct answer is A: Entamoeba histolytica. This pathogen is known to cause amoebic dysentery, characterized by diarrhea with bloody or mucoid stools. The presence of cysts with four nuclei in the stool microscopy is a characteristic feature of Entamoeba histolytica. Other choices are incorrect because Giardia lamblia typically presents with binucleate cysts, Balantidium coli is a ciliated protozoan, and Trichomonas hominis is a non-pathogenic parasite found in the human intestine.