Bacteriophages reproduce by:
- A. Division
- B. Mitosis
- C. Meiosis
- D. None of them
Correct Answer: D
Rationale: Bacteriophages are viruses that infect bacteria, not living organisms. They do not reproduce through division, mitosis, or meiosis because they lack the cellular machinery for these processes. Instead, bacteriophages replicate by inserting their genetic material into a host bacterium and hijacking its cellular machinery to produce new viral particles. Therefore, the correct answer is D - None of them. Division and mitosis involve cellular division in living organisms, while meiosis is a specialized process for sexual reproduction in eukaryotic organisms, none of which apply to bacteriophages.
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The most common bacterial nonsexually transmitted infection of the male reproductive system is:
- A. cystitis
- B. prostatitis
- C. mastitis
- D. nephritis
Correct Answer: B
Rationale: The correct answer is B: prostatitis. Prostatitis is the most common bacterial infection affecting the male reproductive system. It is characterized by inflammation of the prostate gland due to bacterial infection. Symptoms include pain in the pelvic area, difficulty urinating, and fever. Cystitis (A) is a bladder infection, not specific to the male reproductive system. Mastitis (C) is inflammation of the breast tissue, which is not related to the male reproductive system. Nephritis (D) is inflammation of the kidneys, which is also not specific to the male reproductive system.
The eukaryotes known for the presence of a macronucleus and a micronucleus are:
- A. algae
- B. fungi
- C. protozoans
- D. slime molds
Correct Answer: C
Rationale: The correct answer is C: protozoans. Protozoans are eukaryotic organisms that possess both a macronucleus, responsible for general cell functions, and a micronucleus, involved in genetic processes like reproduction. Algae (choice A) typically have a single nucleus, fungi (choice B) lack distinct macronucleus and micronucleus structures, and slime molds (choice D) do not have the same nucleus organization as protozoans. Thus, protozoans are the only group among the choices that exhibit the specific characteristics of having both a macronucleus and a micronucleus.
Which bacteria are capable of forming spores to survive in extreme conditions?
- A. Clostridium botulinum
- B. Bacillus anthracis
- C. Both A and B
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C because both Clostridium botulinum and Bacillus anthracis are capable of forming spores to survive in extreme conditions. Clostridium botulinum is known for causing botulism and produces highly heat-resistant spores, while Bacillus anthracis is the causative agent of anthrax and also forms spores. Choice A is incorrect because only Clostridium botulinum is mentioned, not Bacillus anthracis. Choice B is incorrect because only Bacillus anthracis is mentioned, not Clostridium botulinum. Choice D is incorrect as it states that none of the bacteria can form spores, which is false based on the characteristics of Clostridium botulinum and Bacillus anthracis.
In 8 days after a surgery the patient developed tetanus. The surgeon suspects this condition to be caused by suture material contaminated by tetanus agent. The material is delivered to a bacteriological laboratory. What nutrient medium is required for primary inoculation of the suture material?
- A. Kitt-Tarozzi medium.
- B. Endo agar.
- C. Sabouraud agar.
- D. Egg-yolk salt agar.
Correct Answer: A
Rationale: The correct answer is A: Kitt-Tarozzi medium. This medium is used for the primary inoculation of suture material suspected of causing tetanus. The rationale is that Kitt-Tarozzi medium contains the necessary nutrients to support the growth of Clostridium tetani, the bacterium responsible for tetanus. This medium is selective and helps in isolating the specific pathogen from the contaminated suture material.
Summary of other choices:
B: Endo agar is used for the isolation of Gram-negative bacteria from clinical and non-clinical samples.
C: Sabouraud agar is used for the isolation of fungi and yeast.
D: Egg-yolk salt agar is used for the detection of lipase-producing bacteria, particularly Staphylococcus aureus.
Microbiological analysis of medicinal raw materials revealed capsular bacteria. What stain method was used to detect the capsules?
- A. Gin's
- B. Ziehl-Neelsen's
- C. Neisser's
- D. Gram's
Correct Answer: A
Rationale: The correct answer is A: Gin's stain method. Gin's stain method is specifically used to detect capsules of bacteria. This method involves staining the bacteria with crystal violet and copper sulfate, which highlights the capsules as a clear halo around the stained bacterial cells. Ziehl-Neelsen's stain is used for acid-fast bacteria like Mycobacterium tuberculosis, Neisser's stain is for detecting Neisseria species, and Gram's stain is for differentiating bacteria based on their cell wall composition (Gram-positive vs. Gram-negative). Therefore, choices B, C, and D are incorrect for this scenario.