DNA replication occurs in which phase of the cell cycle?
- A. G phase
- B. Prophase
- C. Metaphase
- D. S phase
Correct Answer: D
Rationale: The correct answer is D: S phase. During the S phase of the cell cycle, DNA replication takes place to ensure each daughter cell receives a complete set of genetic material. This phase is characterized by the synthesis of new DNA strands. In contrast, choices A, B, and C (G phase, Prophase, Metaphase) are not associated with DNA replication. The G phase is a period of cell growth, Prophase is the initial stage of mitosis, and Metaphase is when chromosomes align. Therefore, the S phase is the specific stage where DNA replication occurs, making it the correct answer.
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Which bacteria is responsible for causing cholera?
- A. Shigella dysenteriae
- B. Vibrio cholerae
- C. Salmonella enterica
- D. Clostridium perfringens
Correct Answer: B
Rationale: The correct answer is B: Vibrio cholerae. Cholera is caused by this specific bacterium due to its production of cholera toxin, leading to severe watery diarrhea. Shigella dysenteriae causes dysentery, Salmonella enterica causes salmonellosis, and Clostridium perfringens causes food poisoning, none of which present with the characteristic symptoms of cholera.
Langerhans cells, which play a role in defense against microbes, are located in the:
- A. epidermis
- B. dermis
- C. hypodermis
- D. subcutaneous layer
Correct Answer: A
Rationale: The correct answer is A: epidermis. Langerhans cells are a type of immune cell found in the epidermis, the outermost layer of the skin. They play a crucial role in the defense against microbes by capturing and presenting antigens to activate the immune system. The dermis (B), hypodermis (C), and subcutaneous layer (D) are located deeper in the skin and do not contain Langerhans cells. Therefore, the epidermis is the correct location for these immune cells to carry out their defensive functions.
Laboratory diagnosis of rabies includes all of these except:
- A. Looking for Negri bodies
- B. Intracerebrally infected mice
- C. Looking for Ernst-Babes granules
- D. Immunofluorescence method
Correct Answer: C
Rationale: The correct answer is C: Looking for Ernst-Babes granules. Laboratory diagnosis of rabies typically involves looking for Negri bodies in the brain tissue, intracerebrally infecting mice for virus isolation, and using immunofluorescence method to detect viral antigens. Ernst-Babes granules are not associated with rabies virus and are not used in the diagnosis of rabies. Therefore, choice C is incorrect.
Which bacterium is known for producing an exotoxin that causes severe diarrhea?
- A. Clostridium tetani
- B. Clostridium botulinum
- C. Vibrio cholerae
- D. Shigella dysenteriae
Correct Answer: C
Rationale: Rationale:
1. Vibrio cholerae produces cholera toxin causing severe diarrhea.
2. Clostridium tetani causes tetanus, not diarrhea.
3. Clostridium botulinum causes botulism, not diarrhea.
4. Shigella dysenteriae causes dysentery, not typical diarrhea.
Summary:
A: Incorrect, causes tetanus.
B: Incorrect, causes botulism.
C: Correct, produces cholera toxin causing severe diarrhea.
D: Incorrect, causes dysentery.
For isolating Rickettsia, the sample is investigated via:
- A. Skin-allergy tests
- B. Serological methods for proving Ab
- C. Chick embryos, cell cultures, and lab animals
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C because Rickettsia requires living cells for growth and replication. Chick embryos, cell cultures, and lab animals provide a suitable environment for Rickettsia isolation. Skin-allergy tests (A) and serological methods for proving antibodies (B) are not directly used for isolating Rickettsia as they do not support the growth of the bacteria. "None of the above" (D) is incorrect as option C involves the appropriate methods for isolating Rickettsia.