McGraw Hill Microbiology Test Bank Related

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Specimen of a patient's sputum was stained with the following dyes and reagents: Ziehl's solution, methylene blue solution, 5% solutoin of sulfuric acid. What staining method was applied?

  • A. Ziehl-Neelsen
  • B. Burri's
  • C. Gram's
  • D. Peshkov's
Correct Answer: A

Rationale: The correct answer is A: Ziehl-Neelsen staining method was applied.
1. Ziehl's solution is used as a primary stain in Ziehl-Neelsen staining for acid-fast organisms like Mycobacterium tuberculosis.
2. Sulfuric acid is used as a decolorizer to remove excess stain.
3. Methylene blue is not part of the Ziehl-Neelsen staining method.
4. Burri's, Gram's, and Peshkov's staining methods are not used for acid-fast staining.