microbiology chapter 12 test bank Related

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The basic requirements of PCR reaction include:

  • A. DNA segment to be amplified
  • B. Two oligonucleotide primers
  • C. Heat stable DNA
  • D. All of these
Correct Answer: D

Rationale: The correct answer is D because all the options are essential for a PCR reaction.
A: DNA segment is required as the template for amplification.
B: Two oligonucleotide primers are needed to initiate DNA replication.
C: Heat stable DNA polymerase (not just heat stable DNA) is crucial for withstanding the high temperatures during PCR.
In summary, without any of these components, the PCR reaction cannot proceed effectively.