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When examining a patient presumptively diagnosed with food toxicoinfection, a doctor on duty has detected symptoms characteristic of botulism. The patient named the meals he ate the day before. What is the most probable cause of infection?
- A. Homemade canned meat
- B. Custard pastry from a private bakery
- C. Sour cream from a local dairy factory
- D. Strawberries from a suburban vegetable garden
Correct Answer: A
Rationale: The correct answer is A: Homemade canned meat. Botulism is caused by the Clostridium botulinum bacteria, which thrives in low-oxygen environments like improperly canned food. Homemade canned meat poses a higher risk of contamination as it may not have been processed correctly, allowing the bacteria to grow and produce toxins. Custard pastry, sour cream, and strawberries are less likely to cause botulism as they are not typically associated with the bacteria unless improperly handled or stored. Homemade canned meat is the most probable cause based on the characteristic symptoms of botulism and the conditions that favor the growth of Clostridium botulinum in canned food.
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Algae that contain agar in their cell walls belong to:
- A. Chrysophyta
- B. Rhodophyta
- C. Chlorophyta
- D. Phaeophyta
Correct Answer: B
Rationale: The correct answer is B: Rhodophyta. Rhodophyta is the phylum of red algae, which are known to contain agar in their cell walls. Agar is a gelatinous substance derived from these algae. Chrysophyta (A), Chlorophyta (C), and Phaeophyta (D) do not typically contain agar in their cell walls. Chrysophyta includes diatoms, Chlorophyta includes green algae, and Phaeophyta includes brown algae, none of which are known for agar production. Therefore, Rhodophyta is the correct choice based on the presence of agar in their cell walls.
Which of the following bacteria causes the disease diphtheria?
- A. Bordetella pertussis
- B. Corynebacterium diphtheriae
- C. Streptococcus pyogenes
- D. Neisseria meningitidis
Correct Answer: B
Rationale: The correct answer is B: Corynebacterium diphtheriae. This bacterium produces a toxin that causes diphtheria by inhibiting protein synthesis, leading to the formation of a pseudomembrane in the throat. Bordetella pertussis causes whooping cough, Streptococcus pyogenes causes strep throat, and Neisseria meningitidis causes meningitis. Corynebacterium diphtheriae is the only bacterium among the choices known to cause diphtheria due to its specific toxin production.
Production of RNA from DNA is called
- A. Transposition
- B. Translation
- C. Transcription
- D. RNA splicing
Correct Answer: C
Rationale: The correct answer is C: Transcription. Transcription is the process of synthesizing RNA from a DNA template. During transcription, RNA polymerase binds to the DNA and produces a complementary RNA strand. This process is essential for gene expression.
Explanation for why other choices are incorrect:
A: Transposition involves the movement of genetic elements within a genome, not the production of RNA from DNA.
B: Translation is the process of synthesizing proteins from mRNA, not the production of RNA from DNA.
D: RNA splicing is the process of removing introns and joining exons in pre-mRNA to produce mature mRNA, not the initial production of RNA from DNA.
Acute hemorrhagic cystitis may be due to:
- A. Parvovirus B19
- B. CMV
- C. Adenovirus
- D. Herpesvirus type 2
Correct Answer: C
Rationale: Rationale for choice C (Adenovirus) being correct:
1. Adenovirus is a common cause of acute hemorrhagic cystitis.
2. Adenovirus infects the bladder epithelium, leading to inflammation and bleeding.
3. Symptoms include hematuria and bladder pain.
4. Diagnosis is confirmed through viral culture or PCR testing.
Summary of why other choices are incorrect:
A: Parvovirus B19 - More commonly associated with erythema infectiosum (fifth disease) and aplastic anemia.
B: CMV - Known to cause congenital infections, mononucleosis, and retinitis, not typically associated with hemorrhagic cystitis.
D: Herpesvirus type 2 - Commonly causes genital herpes, but not typically associated with acute hemorrhagic cystitis.
A patient has a necrotizing phlegmon of his lower extremity. A doctor suspects a gas gangrene. Microscopy reveals grampositive bacilli. In order to confirm the diagnosis further bacteriological tests should include inoculation of the material into the following nutrient medium:
- A. Kitt-Tarozzi medium
- B. Endo agar
- C. Meat-peptone agar
- D. Levine agar
Correct Answer: A
Rationale: The correct answer is A: Kitt-Tarozzi medium. This medium is specifically designed to culture Clostridium species, the causative agents of gas gangrene. Clostridium species are gram-positive bacilli that produce gas during their metabolism. Inoculating the material into Kitt-Tarozzi medium allows for the selective growth of Clostridium species, aiding in the confirmation of a gas gangrene diagnosis.
Other choices are incorrect:
B: Endo agar is used for the isolation of gram-negative enteric bacteria.
C: Meat-peptone agar is a general-purpose medium and not specific for Clostridium species.
D: Levine agar is used for the isolation of Salmonella and Shigella species, not Clostridium.