Which of the following organisms is a common cause of peptic ulcers?
- A. Treponema pallidum
- B. Vibrio cholerae
- C. Helicobacter pylori
- D. Escherichia coli
Correct Answer: C
Rationale: The correct answer is C: Helicobacter pylori. This bacterium is a well-known cause of peptic ulcers due to its ability to survive in the acidic environment of the stomach and produce enzymes that weaken the protective mucous layer of the stomach lining. Treponema pallidum (choice A) causes syphilis, Vibrio cholerae (choice B) causes cholera, and Escherichia coli (choice D) can cause various infections but is not a common cause of peptic ulcers. Therefore, Helicobacter pylori is the correct answer as it is specifically associated with peptic ulcer formation.
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A patient with pneumonia had sputum cultured, revealing Gram-negative rods. The bacteria were lactose fermenters and produced green colonies on MacConkey agar. What is the most likely causative agent?
- A. Escherichia coli
- B. Klebsiella pneumoniae
- C. Pseudomonas aeruginosa
- D. Proteus mirabilis
Correct Answer: A
Rationale: Rationale:
1. Gram-negative rods suggest Enterobacteriaceae family.
2. Lactose fermenters narrow down to E. coli.
3. Green colonies on MacConkey agar indicate acid production.
4. E. coli is a common cause of pneumonia.
Therefore, the most likely causative agent is Escherichia coli. Other choices are incorrect as Klebsiella is non-lactose fermenter, Pseudomonas is oxidase-positive, and Proteus is non-lactose fermenter.
A wound swab from a patient with a deep tissue infection revealed Gram-positive rods with terminal spores. What is the most likely causative agent?
- A. Clostridium perfringens
- B. Bacillus anthracis
- C. Clostridium tetani
- D. Bacillus cereus
Correct Answer: A
Rationale: The correct answer is A: Clostridium perfringens. This is because Clostridium perfringens is a Gram-positive rod bacterium that forms terminal spores. It is commonly associated with deep tissue infections and can cause gas gangrene. Bacillus anthracis (B) causes anthrax and does not typically form spores at the terminal end. Clostridium tetani (C) causes tetanus and Bacillus cereus (D) is more commonly associated with food poisoning. In this case, the presence of terminal spores along with the clinical presentation of a deep tissue infection points towards Clostridium perfringens as the most likely causative agent.
A bacterial colony is:
- A. Visible formation, formed by clustering of cells, belonging to different bacterial species
- B. Formed only on a liquid nutrient medium
- C. Both are incorrect
- D. None of the above
Correct Answer: A
Rationale: Step 1: A bacterial colony is a visible formation: Correct as colonies are visible to the naked eye.
Step 2: Formed by clustering of cells: Correct as colonies are made up of numerous bacterial cells.
Step 3: Belonging to different bacterial species: Correct as colonies can consist of one or multiple species.
Summary:
Choice A is correct as it accurately describes a bacterial colony. Choice B is incorrect as colonies can also form on solid nutrient mediums. Choice C is incorrect as choice A is correct. Choice D is incorrect as there is a correct option provided.
During the skill-building session in microbiology the students need to stain the prepared and fixed sputum smears obtained from a tuberculosis patient. What staining technique should be used in this case?
- A. Gram
- B. Giemsa
- C. Ziehl-Neelsen
- D. Burri
Correct Answer: C
Rationale: The correct staining technique for sputum smears obtained from a tuberculosis patient is Ziehl-Neelsen staining. This technique uses carbol fuchsin dye to stain the acid-fast bacteria, such as Mycobacterium tuberculosis, red. Here's a step-by-step rationale:
1. Ziehl-Neelsen staining is specifically designed to detect acid-fast bacteria like Mycobacterium tuberculosis.
2. Carbol fuchsin dye is used to penetrate the mycolic acid layer in the cell wall of the bacteria, making them resistant to decolorization.
3. Acid-alcohol is used as a decolorizer to remove the stain from non-acid-fast bacteria.
4. Methylene blue is used as a counterstain to visualize non-acid-fast bacteria in blue.
Therefore, Ziehl-Neelsen staining is the most appropriate technique for identifying Mycobacterium tuberculosis in sputum smears.
Other choices like Gram, Giemsa, and
A wound smear revealed Gram-negative rods producing blue-green pigment. The bacteria had a fruity odor. What is the causative agent?
- A. Pseudomonas aeruginosa
- B. Escherichia coli
- C. Klebsiella pneumoniae
- D. Proteus mirabilis
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. Pseudomonas aeruginosa is known to produce a blue-green pigment called pyocyanin, which is characteristic of the organism. Additionally, it has a distinct fruity odor due to the production of certain volatile compounds. Escherichia coli (B) does not produce blue-green pigment or have a fruity odor. Klebsiella pneumoniae (C) and Proteus mirabilis (D) also do not exhibit these specific characteristics associated with Pseudomonas aeruginosa.