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A man died from an acute infectious disease accompanied by fever, jaundice, haemorrhagic rash on the skin and mucous membranes as well as by acute renal insufficiency. Histological examination of renal tissue (stained by Romanovsky-Giemsa method) revealed some convoluted bacteria looking like C and S letters. What bacteria were revealed?
- A. Leptospira
- B. Treponema
- C. Spirilla
- D. Borrelia
Correct Answer: A
Rationale: The correct answer is A: Leptospira. The key features pointing to Leptospira are the presence of convoluted bacteria resembling C and S letters, which is characteristic of Leptospira species. Leptospirosis presents with fever, jaundice, haemorrhagic rash, and acute renal insufficiency. The other choices (B: Treponema, C: Spirilla, D: Borrelia) do not match the histological description or the clinical presentation of the case. Treponema is associated with syphilis, Spirilla with genus Spirochaeta, and Borrelia with Lyme disease, none of which match the given scenario.
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Which bacteria is responsible for causing the disease known as whooping cough?
- A. Clostridium botulinum
- B. Bordetella pertussis
- C. Streptococcus pneumoniae
- D. Neisseria meningitidis
Correct Answer: B
Rationale: The correct answer is B: Bordetella pertussis. This bacterium causes whooping cough by infecting the respiratory tract. It produces toxins that lead to severe coughing fits characteristic of the disease. Clostridium botulinum (A) causes botulism, Streptococcus pneumoniae (C) causes pneumonia, and Neisseria meningitidis (D) causes meningitis, not whooping cough. B. pertussis is the only bacterium specifically associated with whooping cough.
A patient underwent esophagogastroduodenoscopy. Analysis of the biopsy material enabled doctors to diagnose him with helicobacteriosis. What property of the bacteria found in this patient had to be obligatory taken into account during their cultivation?
- A. Microaerophilic ability
- B. Presence of urease
- C. Absence of spores and capsules
- D. Colonisation of gastral cells
Correct Answer: A
Rationale: Step-by-step rationale for why choice A is correct:
1. Helicobacter pylori, the bacteria causing helicobacteriosis, is microaerophilic, requiring low oxygen levels.
2. Cultivation conditions must mimic the natural environment of the bacteria.
3. Failure to provide a microaerophilic environment can hinder bacterial growth.
4. Thus, the ability to cultivate these bacteria requires consideration of their microaerophilic nature.
Summary:
- Choice A is correct because Helicobacter pylori is a microaerophilic bacteria.
- Choice B (Presence of urease) is incorrect as it is a characteristic used for diagnosis, not cultivation.
- Choice C (Absence of spores and capsules) is incorrect as it does not relate to cultivation requirements.
- Choice D (Colonization of gastral cells) is incorrect as it pertains to bacterial behavior, not cultivation needs.
Microscopic examination of a microbial culture revealed fusiform spore-forming microorganisms that get violet-blue Gram's stain. What microorganisms were revealed?
- A. Clostridia
- B. Streptococci
- C. Spirochaete
- D. Actinomycete
Correct Answer: A
Rationale: The correct answer is A: Clostridia. Fusiform spore-forming microorganisms that stain violet-blue with Gram's stain are characteristic of Clostridia. Clostridia are anaerobic, rod-shaped bacteria known for their ability to form spores and cause diseases such as tetanus and botulism.
Summary of other choices:
B: Streptococci are spherical, not fusiform, and typically do not form spores.
C: Spirochaete are spiral-shaped bacteria and do not typically form spores.
D: Actinomycete are filamentous bacteria that do not typically form spores and do not stain violet-blue with Gram's stain.
Differentiating media are:
- A. Lowenstein-Jensen and Petraniani
- B. Apoholate-citrate aga and Brilliant-green phenol-red Lactose Agar
- C. Endo, Gassner and Levin
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C because Endo, Gassner, and Levin are all examples of differential media used in microbiology. These media contain specific components that allow for the differentiation of different types of microorganisms based on their metabolic characteristics or biochemical reactions. Lowenstein-Jensen and Petraniani (choice A) are selective media for mycobacteria, not differential. Apoholate-citrate agar and Brilliant-green phenol-red Lactose agar (choice B) are selective media for Salmonella and E. coli, respectively, not differential. Therefore, the correct choice is C as it includes examples of differential media commonly used for microbial identification.
The main structure components of Viruses are:
- A. Glycoproteins and proteins
- B. Nucleic acids and lipids
- C. Nucleic acid and proteins
- D. Lipids and proteins
Correct Answer: C
Rationale: The correct answer is C: Nucleic acid and proteins. Viruses contain genetic material in the form of nucleic acids (DNA or RNA) surrounded by a protein coat. This genetic material carries instructions for viral replication. Proteins are also essential components of viruses, serving various functions like facilitating viral entry into host cells. Option A is incorrect as lipids are not main components of viruses. Option B is incorrect as lipids are not typically found in the main structure of viruses. Option D is incorrect as lipids are not primary components of viruses, and glycoproteins are not always present in the main structure of viruses.