The cytoplasmic membrane in bacteria is composed of:
- A. Phospholipids, glycerol and glycolipids
- B. Lipopolysaccharides
- C. Lipopolysaccharides and peptidoglycan
- D. None of the above
Correct Answer: A
Rationale: The correct answer is A because the cytoplasmic membrane in bacteria is primarily composed of phospholipids, glycerol, and glycolipids. Phospholipids form the basic structure of the membrane, with glycerol serving as the backbone and glycolipids present on the outer surface. Lipopolysaccharides (choice B) are primarily found in the outer membrane of Gram-negative bacteria, not the cytoplasmic membrane. Choice C is incorrect because peptidoglycan is a component of the bacterial cell wall, not the cytoplasmic membrane. Choice D is incorrect as the cytoplasmic membrane in bacteria is indeed composed of phospholipids, glycerol, and glycolipids.
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DNA replication occurs in which phase of the cell cycle?
- A. G phase
- B. Prophase
- C. Metaphase
- D. S phase
Correct Answer: D
Rationale: The correct answer is D: S phase. During the S phase of the cell cycle, DNA replication takes place to ensure each daughter cell receives a complete set of genetic material. This phase is characterized by the synthesis of new DNA strands. In contrast, choices A, B, and C (G phase, Prophase, Metaphase) are not associated with DNA replication. The G phase is a period of cell growth, Prophase is the initial stage of mitosis, and Metaphase is when chromosomes align. Therefore, the S phase is the specific stage where DNA replication occurs, making it the correct answer.
A wound culture revealed Gram-negative rods that are lactose non-fermenters. The bacteria were isolated from a patient with a deep tissue infection. What is the most likely microorganism?
- A. Pseudomonas aeruginosa
- B. Escherichia coli
- C. Salmonella typhi
- D. Shigella dysenteriae
Correct Answer: A
Rationale: The correct answer is A: Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative rod that is a lactose non-fermenter commonly associated with deep tissue infections. It is an opportunistic pathogen known for its ability to cause infections in immunocompromised individuals. P. aeruginosa is often resistant to multiple antibiotics and can form biofilms, making it difficult to treat.
Summary of why other choices are incorrect:
B: Escherichia coli is a Gram-negative rod that ferments lactose and is commonly found in the gastrointestinal tract. It is not typically associated with deep tissue infections.
C: Salmonella typhi is a Gram-negative rod that causes typhoid fever, primarily affecting the gastrointestinal system, not deep tissue infections.
D: Shigella dysenteriae is a Gram-negative rod that causes bacillary dysentery, which primarily affects the gastrointestinal tract and does not typically cause deep tissue infections.
While studying a microslide obtained from the punctuate of a regional lymph node and stained by Romanovsky-Giemsa method a physician revealed some light pink thin microorganisms with 12-14 regular spiral coils and pointed ends, up to 10-13 micrometer long. This might be the causative agent of the following disease:
- A. Syphilis
- B. Trypanosomiasis
- C. Leptospirosis
- D. Relapsing fever
Correct Answer: D
Rationale: The correct answer is D: Relapsing fever. The description matches Borrelia species, the causative agent of relapsing fever. The thin, spiral-shaped microorganisms with pointed ends and specific length are characteristic of Borrelia. The number of coils and size mentioned also align with Borrelia morphology. Relapsing fever is transmitted by tick bites and manifests as recurring febrile episodes.
Incorrect choices:
A: Syphilis - Syphilis is caused by Treponema pallidum, which is not spiral-shaped.
B: Trypanosomiasis - Trypanosomiasis is caused by Trypanosoma species, which are not spiral-shaped.
C: Leptospirosis - Leptospirosis is caused by Leptospira species, which are not spiral-shaped and do not match the specific description provided.
For isolating Rickettsia, the sample is investigated via:
- A. Skin-allergy tests
- B. Serological methods for proving Ab
- C. Chick embryos, cell cultures, and lab animals
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C because Rickettsia requires living cells for growth and replication. Chick embryos, cell cultures, and lab animals provide a suitable environment for Rickettsia isolation. Skin-allergy tests (A) and serological methods for proving antibodies (B) are not directly used for isolating Rickettsia as they do not support the growth of the bacteria. "None of the above" (D) is incorrect as option C involves the appropriate methods for isolating Rickettsia.
Which bacterium is known for producing an exotoxin that causes severe diarrhea?
- A. Clostridium tetani
- B. Clostridium botulinum
- C. Vibrio cholerae
- D. Shigella dysenteriae
Correct Answer: C
Rationale: Rationale:
1. Vibrio cholerae produces cholera toxin causing severe diarrhea.
2. Clostridium tetani causes tetanus, not diarrhea.
3. Clostridium botulinum causes botulism, not diarrhea.
4. Shigella dysenteriae causes dysentery, not typical diarrhea.
Summary:
A: Incorrect, causes tetanus.
B: Incorrect, causes botulism.
C: Correct, produces cholera toxin causing severe diarrhea.
D: Incorrect, causes dysentery.