The term “zone of inhibition†is used in which of the following procedures?
- A. Use-dilution test
- B. Growth inhibition test
- C. Disk-diffusion test
- D. Multiple inhibition test
Correct Answer: C
Rationale: The correct answer is C: Disk-diffusion test. In the disk-diffusion test, the zone of inhibition refers to the area around an antimicrobial disk where bacterial growth is inhibited. This test is used to determine the susceptibility of bacteria to specific antibiotics. The other choices, such as A: Use-dilution test, B: Growth inhibition test, and D: Multiple inhibition test, do not specifically involve measuring the zone of inhibition and are not commonly used methods for testing antibiotic susceptibility.
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The class of immunoglobulins most abundant in body is:
- A. IgG
- B. IgE
- C. IgD
- D. IgM
Correct Answer: A
Rationale: The correct answer is A: IgG. IgG is the most abundant class of immunoglobulins in the body, constituting about 75% of total immunoglobulins. IgG plays a crucial role in long-term immunity, neutralizing toxins, and enhancing phagocytosis. IgE is involved in allergic reactions, IgD is mainly found on B cells and functions as a receptor, and IgM is the first antibody produced in response to an infection but is less abundant compared to IgG. Therefore, IgG is the correct choice based on its abundance and diverse functions in the immune system.
Which of the following bacteria is known for causing the disease known as whooping cough?
- A. Bordetella pertussis
- B. Clostridium tetani
- C. Haemophilus influenzae
- D. Staphylococcus aureus
Correct Answer: A
Rationale: The correct answer is A: Bordetella pertussis. This bacterium is known for causing whooping cough due to its ability to produce toxins that damage the respiratory tract, leading to severe coughing fits. Clostridium tetani causes tetanus, Haemophilus influenzae can cause respiratory infections but not whooping cough, and Staphylococcus aureus is more commonly associated with skin infections. Therefore, A is the correct choice based on its specific association with the disease in question.
Which bacteria are known for causing food poisoning due to their production of enterotoxins?
- A. Clostridium botulinum
- B. Salmonella enterica
- C. Staphylococcus aureus
- D. All of the above
Correct Answer: D
Rationale: The correct answer is D, "All of the above." Clostridium botulinum produces botulinum toxin causing botulism. Salmonella enterica can produce enterotoxins causing food poisoning. Staphylococcus aureus also produces enterotoxins leading to food poisoning. Therefore, all three options can cause food poisoning due to their production of enterotoxins.
The structure used for bacterial motility is:
- A. Fimbriae
- B. Capsule
- C. Flagella
- D. Pili
Correct Answer: C
Rationale: The correct answer is C: Flagella. Flagella are the structures used for bacterial motility, allowing bacteria to move towards or away from stimuli. They are long, whip-like appendages that rotate to propel the bacteria. Fimbriae (A) are short, hair-like structures used for attachment, not movement. Capsules (B) are protective layers surrounding bacteria. Pili (D) are used for adherence and conjugation, not motility. In summary, flagella are specifically designed for bacterial movement, making them the correct choice.
A child with diarrhea had stool microscopy revealing cysts with four nuclei. What is the most likely causative agent?
- A. Entamoeba histolytica
- B. Giardia lamblia
- C. Balantidium coli
- D. Trichomonas hominis
Correct Answer: A
Rationale: The correct answer is A: Entamoeba histolytica. This pathogen is known to cause amoebic dysentery, characterized by diarrhea with bloody or mucoid stools. The presence of cysts with four nuclei in the stool microscopy is a characteristic feature of Entamoeba histolytica. Other choices are incorrect because Giardia lamblia typically presents with binucleate cysts, Balantidium coli is a ciliated protozoan, and Trichomonas hominis is a non-pathogenic parasite found in the human intestine.
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