Which bacteria are associated with causing foodborne infections?
- A. Clostridium botulinum
- B. Escherichia coli
- C. Salmonella enterica
- D. All of the above
Correct Answer: D
Rationale: The correct answer is D (All of the above) because all three bacteria (Clostridium botulinum, Escherichia coli, Salmonella enterica) are known to cause foodborne infections. Clostridium botulinum can lead to botulism, E. coli can cause various gastrointestinal illnesses, and Salmonella enterica is a common cause of food poisoning. Each bacterium has specific mechanisms that result in foodborne illnesses. Choices A, B, and C are incorrect because each bacterium mentioned is associated with causing foodborne infections, making them collectively the correct answer.
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When using gram staining method fungi stain:
- A. Blue-purple
- B. Red
- C. Silver
- D. Do not stain
Correct Answer: D
Rationale: The correct answer is D: Fungi do not stain in the gram staining method because they have a unique cell wall composition different from bacteria. Fungi have chitin in their cell walls, which is not easily stained by the gram staining process. This method primarily targets the peptidoglycan layer present in bacterial cell walls. Therefore, fungi will not take up the stain and will remain colorless. The other choices (A: Blue-purple, B: Red, C: Silver) are incorrect because they describe colors that are associated with staining results for bacteria, not fungi.
Which bacteria is responsible for causing leprosy?
- A. Streptococcus pyogenes
- B. Mycobacterium leprae
- C. Neisseria gonorrhoeae
- D. Escherichia coli
Correct Answer: B
Rationale: The correct answer is B: Mycobacterium leprae. This bacterium is responsible for causing leprosy, also known as Hansen's disease. Mycobacterium leprae specifically targets the skin, peripheral nerves, and mucosa of the upper respiratory tract. It is an acid-fast bacterium with a slow growth rate, making it difficult to culture in the laboratory. Streptococcus pyogenes (choice A) is responsible for various infections such as strep throat, Neisseria gonorrhoeae (choice C) causes gonorrhea, and Escherichia coli (choice D) is commonly found in the gut and can lead to various infections but not leprosy.
A defined microorganism can be isolated in pure culture:
- A. From liquid and solid media
- B. From liquid nutrient media
- C. Only from single colonies on solid media
- D. None of the above
Correct Answer: C
Rationale: The correct answer is C because isolating a defined microorganism in pure culture involves obtaining a single, distinct colony on solid media. This ensures that only one type of microorganism is present in the culture, preventing contamination. Liquid media can harbor multiple organisms, making it challenging to isolate a specific microorganism. Therefore, choice A is incorrect. Choice B is also incorrect as liquid nutrient media does not provide the necessary isolation required for pure culture. Choice D is incorrect because isolating from single colonies on solid media allows for the pure culture of the defined microorganism.
Which structure in bacteria is used for attachment to surfaces?
- A. Flagella
- B. Fimbriae
- C. Pili
- D. Capsule
Correct Answer: B
Rationale: The correct answer is B: Fimbriae. Fimbriae are short, hair-like appendages on the surface of bacteria that help in attachment to surfaces. They aid in colonization and biofilm formation. Flagella (A) are used for motility. Pili (C) are longer appendages used for conjugation and adherence to other cells. Capsules (D) are protective structures that aid in evading the host immune system, not necessarily for attachment.
Which scientist performed the first successful vaccination against smallpox?
- A. Pasteur
- B. Jenner
- C. Semmelweis
- D. Koch
Correct Answer: B
Rationale: The correct answer is B: Jenner. Edward Jenner is credited with performing the first successful vaccination against smallpox in 1796. He used cowpox virus to inoculate individuals, leading to immunity against smallpox. Jenner's experiment laid the foundation for modern vaccination. Pasteur (A) is known for developing the germ theory of disease. Semmelweis (C) advocated for handwashing to prevent infections. Koch (D) identified the causative agents of several diseases but did not develop the smallpox vaccine.